Skip to main content

Inflammatory cells degrade inter-alpha inhibitor to liberate urinary proteinase inhibitors.

Publication ,  Journal Article
Pratt, CW; Swaim, MW; Pizzo, SV
Published in: J Leukoc Biol
January 1989

The relationship between inter-alpha inhibitor (I alpha I) and urinary proteinase inhibitor (UPI) was examined by comparing purified UPI with a proteolytic fragment of I alpha I (I'), and by demonstrating that inflammatory cells produce similar fragments under physiologic conditions. Purified I', derived by chymotrypsin digestion of I alpha I, was similar to UPI in apparent molecular weight (68,000-69,000), amino acid composition, immunoreactivity, and inhibitory activity against trypsin, chymotrypsin, and neutrophil elastase. The production of similar inhibitory fragments by murine peritoneal macrophages, human neutrophils, and a murine mast cell line was quantified. Neutrophils were most efficient at proteolyzing I alpha I. Comparison of the pattern of I alpha I degradation by neutrophil preparations with that by pure enzymes, suggested that both elastase and cathepsin G mediate neutrophil proteolysis of I alpha I. These proteinases may thus be responsible for inflammation-related increases in UPI-like inhibitor levels in vivo.

Duke Scholars

Published In

J Leukoc Biol

DOI

ISSN

0741-5400

Publication Date

January 1989

Volume

45

Issue

1

Start / End Page

1 / 9

Location

England

Related Subject Headings

  • Neutrophils
  • Mice
  • Mast Cells
  • Macrophages
  • Immunology
  • Humans
  • Glycoproteins
  • Cells, Cultured
  • Animals
  • Alpha-Globulins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pratt, C. W., Swaim, M. W., & Pizzo, S. V. (1989). Inflammatory cells degrade inter-alpha inhibitor to liberate urinary proteinase inhibitors. J Leukoc Biol, 45(1), 1–9. https://doi.org/10.1002/jlb.45.1.1
Pratt, C. W., M. W. Swaim, and S. V. Pizzo. “Inflammatory cells degrade inter-alpha inhibitor to liberate urinary proteinase inhibitors.J Leukoc Biol 45, no. 1 (January 1989): 1–9. https://doi.org/10.1002/jlb.45.1.1.
Pratt CW, Swaim MW, Pizzo SV. Inflammatory cells degrade inter-alpha inhibitor to liberate urinary proteinase inhibitors. J Leukoc Biol. 1989 Jan;45(1):1–9.
Pratt, C. W., et al. “Inflammatory cells degrade inter-alpha inhibitor to liberate urinary proteinase inhibitors.J Leukoc Biol, vol. 45, no. 1, Jan. 1989, pp. 1–9. Pubmed, doi:10.1002/jlb.45.1.1.
Pratt CW, Swaim MW, Pizzo SV. Inflammatory cells degrade inter-alpha inhibitor to liberate urinary proteinase inhibitors. J Leukoc Biol. 1989 Jan;45(1):1–9.

Published In

J Leukoc Biol

DOI

ISSN

0741-5400

Publication Date

January 1989

Volume

45

Issue

1

Start / End Page

1 / 9

Location

England

Related Subject Headings

  • Neutrophils
  • Mice
  • Mast Cells
  • Macrophages
  • Immunology
  • Humans
  • Glycoproteins
  • Cells, Cultured
  • Animals
  • Alpha-Globulins