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Nickel inhibits binding of alpha2-macroglobulin-methylamine to the low-density lipoprotein receptor-related protein/alpha2-macroglobulin receptor but not the alpha2-macroglobulin signaling receptor.

Publication ,  Journal Article
Odom, AR; Misra, UK; Pizzo, SV
Published in: Biochemistry
October 14, 1997

A previous study demonstrated that activated alpha2-macroglobulin (alpha2M*) binding to the low-density receptor-related protein/alpha2-macroglobulin receptor (LRP/alpha2MR) is blocked by Ni2+ [Hussain, M. M., et al. (1995) Biochemistry 34, 16074-16081]. We now report that the effect of Ni2+ is on a region of the alpha2M molecule upstream of the carboxyl terminal receptor recognition domain. This observation is consistent with previous observations from this laboratory suggesting that alpha2M* binding to LRP/alpha2MR involves a region of the alpha2M molecule immediately upstream of the receptor recognition domain [Enghild, J. J., et al. (1989) Biochemistry 28, 1406-1412]. We further demonstrate that Ni2+ has no effect on the binding of alpha2M* or a cloned and expressed receptor binding fragment (RBF) to the recently described alpha2M signaling receptor as assessed by direct binding and signal transduction studies.

Duke Scholars

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

October 14, 1997

Volume

36

Issue

41

Start / End Page

12395 / 12399

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • Signal Transduction
  • Receptors, Immunologic
  • Nickel
  • Mice, Inbred C57BL
  • Mice
  • Methylamines
  • Macrophages
  • Low Density Lipoprotein Receptor-Related Protein-1
  • Escherichia coli
 

Citation

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Odom, A. R., U. K. Misra, and S. V. Pizzo. “Nickel inhibits binding of alpha2-macroglobulin-methylamine to the low-density lipoprotein receptor-related protein/alpha2-macroglobulin receptor but not the alpha2-macroglobulin signaling receptor.Biochemistry 36, no. 41 (October 14, 1997): 12395–99. https://doi.org/10.1021/bi970806k.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

October 14, 1997

Volume

36

Issue

41

Start / End Page

12395 / 12399

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • Signal Transduction
  • Receptors, Immunologic
  • Nickel
  • Mice, Inbred C57BL
  • Mice
  • Methylamines
  • Macrophages
  • Low Density Lipoprotein Receptor-Related Protein-1
  • Escherichia coli