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The effect of divalent cations on the conformation and function of human plasminogen.

Publication ,  Journal Article
Stack, S; Gonzalez-Gronow, M; Pizzo, SV
Published in: Arch Biochem Biophys
January 1991

The activation of native human plasminogen (Glu1-Pg) by tissue plasminogen activator, urinary plasminogen activator (u-PA), and streptokinase is inhibited by the divalent cations Ca2+, Mg2+, and Mn2+. This inhibition is accompanied by a conformational change in the molecule as evidenced by a decrease in Stokes' radius and intrinsic fluorescence. Kinetic analysis indicates that Mn2+ acts as an uncompetitive inhibitor of u-PA-catalyzed Glu1-Pg activation. In contrast to the inhibitory effects of divalent cations on Glu1-Pg, Ca2+ and Mg2+ stimulate the activation of proteolytically modified Lys77-Pg. These observations provide further evidence that Glu1-Pg and Lys77-Pg exhibit differential responses to ligands in the microenvironment.

Duke Scholars

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

January 1991

Volume

284

Issue

1

Start / End Page

58 / 62

Location

United States

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Spectrometry, Fluorescence
  • Protein Conformation
  • Plasminogen
  • Lysine
  • Kinetics
  • In Vitro Techniques
  • Humans
  • Glutamates
  • Enzyme Activation
 

Citation

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Stack, S., Gonzalez-Gronow, M., & Pizzo, S. V. (1991). The effect of divalent cations on the conformation and function of human plasminogen. Arch Biochem Biophys, 284(1), 58–62. https://doi.org/10.1016/0003-9861(91)90263-i
Stack, S., M. Gonzalez-Gronow, and S. V. Pizzo. “The effect of divalent cations on the conformation and function of human plasminogen.Arch Biochem Biophys 284, no. 1 (January 1991): 58–62. https://doi.org/10.1016/0003-9861(91)90263-i.
Stack S, Gonzalez-Gronow M, Pizzo SV. The effect of divalent cations on the conformation and function of human plasminogen. Arch Biochem Biophys. 1991 Jan;284(1):58–62.
Stack, S., et al. “The effect of divalent cations on the conformation and function of human plasminogen.Arch Biochem Biophys, vol. 284, no. 1, Jan. 1991, pp. 58–62. Pubmed, doi:10.1016/0003-9861(91)90263-i.
Stack S, Gonzalez-Gronow M, Pizzo SV. The effect of divalent cations on the conformation and function of human plasminogen. Arch Biochem Biophys. 1991 Jan;284(1):58–62.
Journal cover image

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

January 1991

Volume

284

Issue

1

Start / End Page

58 / 62

Location

United States

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Spectrometry, Fluorescence
  • Protein Conformation
  • Plasminogen
  • Lysine
  • Kinetics
  • In Vitro Techniques
  • Humans
  • Glutamates
  • Enzyme Activation