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Potentiation of calcium levels by extracellular arachidonic acid in nuclei isolated from macrophages stimulated with receptor-recognized forms of alpha(2)-macroglobulin.

Publication ,  Journal Article
Misra, UK; Gawdi, G; Pizzo, SV
Published in: Cell Signal
February 2000

Ligation of macrophage alpha(2)-macroglobulin signalling receptors (alpha(2)MSR) with activated alpha(2)-macroglobulin (alpha(2)M*) increases intracellular Ca(2+), and cytosolic phospholipase A(2) (cPLA(2)) and phospholipase D activities. In view of the relationship between cellular Ca(2+) and mitogenesis, we examined the effect of the product of cPLA(2) activity, arachidonic acid (AA), on nuclear Ca(2+) levels in macrophages stimulated with alpha(2)M*, platelet derived growth factor, and bradykinin. AA addition increased Ca(2+) levels in Fura-2/AM loaded nuclei from both buffer-treated and agonist-stimulated cells, but the increase in stimulated macrophages was 2-4-fold higher. Preincubation of Fura-2/AM loaded nuclei with EGTA or BAPTA/AM abolished AA-induced increase in nuclear Ca(2+) levels. Preincubation of nuclei with indomethacin did not affect AA-induced increase in nuclear Ca(2+) in agonist-stimulated nuclei. It is concluded that in macrophages stimulated with various agonists, AA, derived from cPLA(2)-dependent hydrolysis of phospholipids, plays a significant role in regulating nuclear Ca(2+) levels and thus nuclear functions.

Duke Scholars

Published In

Cell Signal

DOI

ISSN

0898-6568

Publication Date

February 2000

Volume

12

Issue

2

Start / End Page

99 / 104

Location

England

Related Subject Headings

  • alpha-Macroglobulins
  • Thapsigargin
  • Signal Transduction
  • Receptors, Immunologic
  • Platelet-Derived Growth Factor
  • Phospholipases A
  • Phospholipase D
  • Macrophages
  • Low Density Lipoprotein Receptor-Related Protein-1
  • Indomethacin
 

Citation

Journal cover image

Published In

Cell Signal

DOI

ISSN

0898-6568

Publication Date

February 2000

Volume

12

Issue

2

Start / End Page

99 / 104

Location

England

Related Subject Headings

  • alpha-Macroglobulins
  • Thapsigargin
  • Signal Transduction
  • Receptors, Immunologic
  • Platelet-Derived Growth Factor
  • Phospholipases A
  • Phospholipase D
  • Macrophages
  • Low Density Lipoprotein Receptor-Related Protein-1
  • Indomethacin