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An analysis of T cell intrinsic roles of E2A by conditional gene disruption in the thymus.

Publication ,  Journal Article
Pan, L; Hanrahan, J; Li, J; Hale, LP; Zhuang, Y
Published in: J Immunol
April 15, 2002

The importance of E2A transcription factors in T cell development has been demonstrated in studies of E2A-deficient mice, which display abnormal T cell development and a high frequency of T cell lymphomas. Because E2A expression is not restricted to the T cell lineage, the primary cause of the T cell phenotype in E2A-deficient mice was not fully determined. To further investigate the role of E2A in T cell lineage, we generated mice with the E2A gene disrupted exclusively during thymocyte development using the Cre-lox system. We show that this system allows E2A gene disruption to occur throughout the double-negative stage of thymocyte development. E2A deletion appears to be completed before development reaches the double-positive stage. Consistent with the gene disruption, these mice reveal a T cell intrinsic role for E2A during the transition from the double-negative stage to the double-positive stage of thymocyte development. In contrast to germline E2A knockout mice, conditional E2A knockout mice do not develop T cell lymphoma. This work establishes a new model for further investigating E2A function in T cell development and leukemiogenesis.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

April 15, 2002

Volume

168

Issue

8

Start / End Page

3923 / 3932

Location

United States

Related Subject Headings

  • Transcription Factors
  • Thymus Gland
  • T-Lymphocyte Subsets
  • Stromal Cells
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • Helix-Loop-Helix Motifs
  • Gene Targeting
 

Citation

APA
Chicago
ICMJE
MLA
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Pan, L., Hanrahan, J., Li, J., Hale, L. P., & Zhuang, Y. (2002). An analysis of T cell intrinsic roles of E2A by conditional gene disruption in the thymus. J Immunol, 168(8), 3923–3932. https://doi.org/10.4049/jimmunol.168.8.3923
Pan, Lihua, Jenifer Hanrahan, Jie Li, Laura P. Hale, and Yuan Zhuang. “An analysis of T cell intrinsic roles of E2A by conditional gene disruption in the thymus.J Immunol 168, no. 8 (April 15, 2002): 3923–32. https://doi.org/10.4049/jimmunol.168.8.3923.
Pan L, Hanrahan J, Li J, Hale LP, Zhuang Y. An analysis of T cell intrinsic roles of E2A by conditional gene disruption in the thymus. J Immunol. 2002 Apr 15;168(8):3923–32.
Pan, Lihua, et al. “An analysis of T cell intrinsic roles of E2A by conditional gene disruption in the thymus.J Immunol, vol. 168, no. 8, Apr. 2002, pp. 3923–32. Pubmed, doi:10.4049/jimmunol.168.8.3923.
Pan L, Hanrahan J, Li J, Hale LP, Zhuang Y. An analysis of T cell intrinsic roles of E2A by conditional gene disruption in the thymus. J Immunol. 2002 Apr 15;168(8):3923–3932.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

April 15, 2002

Volume

168

Issue

8

Start / End Page

3923 / 3932

Location

United States

Related Subject Headings

  • Transcription Factors
  • Thymus Gland
  • T-Lymphocyte Subsets
  • Stromal Cells
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • Helix-Loop-Helix Motifs
  • Gene Targeting