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Successful laboratory growth and analysis of CUSA-obtained medulloblastoma samples. Technical note.

Publication ,  Journal Article
Oakes, WJ; Friedman, HS; Bigner, SH; Bullock, NH; Bigner, DD
Published in: J Neurosurg
May 1990

The development of the Cavitron ultrasonic surgical aspirator (CUSA) has facilitated neurosurgical intervention for removal of central or peripheral nervous system tumors adjacent to or within vital structures. However, laboratory studies defining the phenotypic and genotypic properties of these tumors, both in cell culture and as xenografts in immunoincompetent animals, require viable tumor fragments free of microbial or red blood cell contamination. This report describes the use of a readily available sterile trap with the CUSA which, in conjunction with centrifugation and ammonium chloride lysis of the bloody aspirate, allowed collection of concentrated viable human medulloblastoma tumor cells. These cells were successfully established in cell culture and as transplantable xenografts in athymic mice.

Duke Scholars

Published In

J Neurosurg

DOI

ISSN

0022-3085

Publication Date

May 1990

Volume

72

Issue

5

Start / End Page

821 / 823

Location

United States

Related Subject Headings

  • Ultrasonic Therapy
  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Suction
  • Specimen Handling
  • Neurology & Neurosurgery
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Oakes, W. J., Friedman, H. S., Bigner, S. H., Bullock, N. H., & Bigner, D. D. (1990). Successful laboratory growth and analysis of CUSA-obtained medulloblastoma samples. Technical note. J Neurosurg, 72(5), 821–823. https://doi.org/10.3171/jns.1990.72.5.0821
Oakes, W. J., H. S. Friedman, S. H. Bigner, N. H. Bullock, and D. D. Bigner. “Successful laboratory growth and analysis of CUSA-obtained medulloblastoma samples. Technical note.J Neurosurg 72, no. 5 (May 1990): 821–23. https://doi.org/10.3171/jns.1990.72.5.0821.
Oakes WJ, Friedman HS, Bigner SH, Bullock NH, Bigner DD. Successful laboratory growth and analysis of CUSA-obtained medulloblastoma samples. Technical note. J Neurosurg. 1990 May;72(5):821–3.
Oakes, W. J., et al. “Successful laboratory growth and analysis of CUSA-obtained medulloblastoma samples. Technical note.J Neurosurg, vol. 72, no. 5, May 1990, pp. 821–23. Pubmed, doi:10.3171/jns.1990.72.5.0821.
Oakes WJ, Friedman HS, Bigner SH, Bullock NH, Bigner DD. Successful laboratory growth and analysis of CUSA-obtained medulloblastoma samples. Technical note. J Neurosurg. 1990 May;72(5):821–823.

Published In

J Neurosurg

DOI

ISSN

0022-3085

Publication Date

May 1990

Volume

72

Issue

5

Start / End Page

821 / 823

Location

United States

Related Subject Headings

  • Ultrasonic Therapy
  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Suction
  • Specimen Handling
  • Neurology & Neurosurgery
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Male