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Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas.

Publication ,  Journal Article
Wikstrand, CJ; Stanley, SD; Humphrey, PA; Pegram, CN; Archer, GE; Kurpad, S; Shibuya, M; Bigner, DD
Published in: J Neuroimmunol
July 1993

We have previously demonstrated antibody production to a glioma-associated variant form of the human epidermal growth factor receptor in rabbits that had received a synthetic peptide mimicking the unique primary structure of the variant protein as immunogen. We report here the response of mice, rabbits, goats, and macaques immunized by various protocols to this peptide. Titers to both peptide- and cell-elaborated variant receptor were measured, and the capacity to recognize the variant receptor in human tumor samples was determined. Within the range of species and strains investigated, we demonstrated a variable species-associated response to the peptide (rabbits > mice > goats > rats > macaques). Rabbits and a single goat produced specific, high titer antibody activity to the variant receptor protein following immunization with peptide alone. Murine titers to the parent protein were not appreciable following peptide immunization alone; additional immunization with variant receptor as expressed on cell membranes was used to boost this response.

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Published In

J Neuroimmunol

DOI

ISSN

0165-5728

Publication Date

July 1993

Volume

46

Issue

1-2

Start / End Page

165 / 173

Location

Netherlands

Related Subject Headings

  • Rabbits
  • Peptides
  • Neurology & Neurosurgery
  • Molecular Sequence Data
  • Mice
  • Macaca
  • Goats
  • Glioma
  • ErbB Receptors
  • Autoantigens
 

Citation

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Wikstrand, C. J., Stanley, S. D., Humphrey, P. A., Pegram, C. N., Archer, G. E., Kurpad, S., … Bigner, D. D. (1993). Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas. J Neuroimmunol, 46(1–2), 165–173. https://doi.org/10.1016/0165-5728(93)90246-u
Wikstrand, C. J., S. D. Stanley, P. A. Humphrey, C. N. Pegram, G. E. Archer, S. Kurpad, M. Shibuya, and D. D. Bigner. “Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas.J Neuroimmunol 46, no. 1–2 (July 1993): 165–73. https://doi.org/10.1016/0165-5728(93)90246-u.
Wikstrand CJ, Stanley SD, Humphrey PA, Pegram CN, Archer GE, Kurpad S, et al. Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas. J Neuroimmunol. 1993 Jul;46(1–2):165–73.
Wikstrand, C. J., et al. “Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas.J Neuroimmunol, vol. 46, no. 1–2, July 1993, pp. 165–73. Pubmed, doi:10.1016/0165-5728(93)90246-u.
Wikstrand CJ, Stanley SD, Humphrey PA, Pegram CN, Archer GE, Kurpad S, Shibuya M, Bigner DD. Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas. J Neuroimmunol. 1993 Jul;46(1–2):165–173.
Journal cover image

Published In

J Neuroimmunol

DOI

ISSN

0165-5728

Publication Date

July 1993

Volume

46

Issue

1-2

Start / End Page

165 / 173

Location

Netherlands

Related Subject Headings

  • Rabbits
  • Peptides
  • Neurology & Neurosurgery
  • Molecular Sequence Data
  • Mice
  • Macaca
  • Goats
  • Glioma
  • ErbB Receptors
  • Autoantigens