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The use of a panel of monoclonal antibodies in the evaluation of cytologic specimens from the central nervous system.

Publication ,  Journal Article
Vick, WW; Wikstrand, CJ; Bullard, DE; Kemshead, J; Coakham, HB; Schlom, J; Johnston, WW; Bigner, DD; Bigner, SH
Published in: Acta Cytol
1987

A panel of monoclonal antibodies was tested immunohistochemically to determine the utility of such reagents in distinguishing among metastatic carcinoma, lymphoma, leukemia and primary brain tumors. The monoclonal antibodies used were: (1) a cocktail comprised of three anti-glial fibrillary acidic protein antibodies (alpha-GFAP); (2) UJ13A, a panneuroectodermal antibody; (3) B72.3, which recognizes a carcinoma-distinctive tumor-associated glycoprotein complex; and (4) 2D1, a pan-leukocyte antibody. Fifty-three specimens (21 cerebrospinal fluids, 1 ventricular fluid, 2 brain cyst fluids, 12 needle washings, 15 imprints, 1 subdural fluid and 1 post-shunt fluid) were obtained from 21 gliomas, 2 meningiomas, 1 pineoblastoma, 11 metastatic tumors, 3 lymphomas, 1 leukemia and 14 cases without tumor. alpha-GFAP stained all 21 gliomas and 5 of 5 cases containing reactive brain fragments. UJ13A had a reactivity pattern similar to that of alpha-GFAP, but also stained the meningiomas, pineoblastoma, oat-cell carcinoma and embryonal rhabdomyosarcoma. B72.3 stained all adenocarcinomas and the large-cell carcinoma. 2D1 stained lymphoma and leukemia, all inflammatory cells and 4 of 12 glioblastomas. Although no single antibody was diagnostic of a specific tumor type, this panel accurately differentiated among most primary brain tumors, metastases, leukemias and lymphomas.

Duke Scholars

Published In

Acta Cytol

ISSN

0001-5547

Publication Date

1987

Volume

31

Issue

6

Start / End Page

815 / 824

Location

Switzerland

Related Subject Headings

  • Pathology
  • Neoplasm Metastasis
  • Middle Aged
  • Lymphoma
  • Leukemia
  • Humans
  • Female
  • Encephalitis
  • Child, Preschool
  • Cerebrospinal Fluid
 

Citation

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Vick, W. W., Wikstrand, C. J., Bullard, D. E., Kemshead, J., Coakham, H. B., Schlom, J., … Bigner, S. H. (1987). The use of a panel of monoclonal antibodies in the evaluation of cytologic specimens from the central nervous system. Acta Cytol, 31(6), 815–824.
Vick, W. W., C. J. Wikstrand, D. E. Bullard, J. Kemshead, H. B. Coakham, J. Schlom, W. W. Johnston, D. D. Bigner, and S. H. Bigner. “The use of a panel of monoclonal antibodies in the evaluation of cytologic specimens from the central nervous system.Acta Cytol 31, no. 6 (1987): 815–24.
Vick WW, Wikstrand CJ, Bullard DE, Kemshead J, Coakham HB, Schlom J, et al. The use of a panel of monoclonal antibodies in the evaluation of cytologic specimens from the central nervous system. Acta Cytol. 1987;31(6):815–24.
Vick WW, Wikstrand CJ, Bullard DE, Kemshead J, Coakham HB, Schlom J, Johnston WW, Bigner DD, Bigner SH. The use of a panel of monoclonal antibodies in the evaluation of cytologic specimens from the central nervous system. Acta Cytol. 1987;31(6):815–824.
Journal cover image

Published In

Acta Cytol

ISSN

0001-5547

Publication Date

1987

Volume

31

Issue

6

Start / End Page

815 / 824

Location

Switzerland

Related Subject Headings

  • Pathology
  • Neoplasm Metastasis
  • Middle Aged
  • Lymphoma
  • Leukemia
  • Humans
  • Female
  • Encephalitis
  • Child, Preschool
  • Cerebrospinal Fluid