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Ganglioside mapping of a human medulloblastoma xenograft.

Publication ,  Journal Article
Gottfries, J; Mansson, JE; Fredman, P; Wikstrand, CJ; Friedman, HS; Bigner, DD; Svennerholm, L
Published in: Acta Neuropathol
1989
Published version (DOI) Link to item

The ganglioside patterns of medulloblastomas have never been established; in this study we report the ganglioside profile of the human medulloblastoma cell line TE-671 grown as a xenograft in nude mice. Gangliosides were isolated and structurally analyzed by fast atom bombardment mass spectometry following permethylation. Identification of individual gangliosides was also performed by immunostaining of high-performance thin-layer chromatography-separated bands. Total ganglioside levels of 0.20 mumol/g of tissue were obtained, consistent with those reported for human glioma cell lines grown as xenografts; predominant monosialogangliosides of TE-671 xenografts were II3-alpha-NeuAc-LacCer (GM3) and II3-alpha-NeuAc-GgOse3Cer (GM2) but there were also relatively large proportions of IV3-alpha-NeuAc-LcOse4Cer (3'-isoLM1), IV3-alpha-NeuAc-nLcOse4Cer (3'-LM1) and a further ganglioside of the neolacto-series with an extra lactosamine moiety. The only oligosialoganglioside detected was IV3, II3-alpha-NeuAc2-GgOse4Cer (GD1a).

Duke Scholars

Darell Doty Bigner
Author Darell Doty Bigner Neurosurgery
Henry Seth Friedman
Author Henry Seth Friedman Neurosurgery, Neuro-Oncology

Published In

Acta Neuropathol

DOI

10.1007/BF00687580

ISSN

0001-6322

Publication Date

1989

Volume

77

Issue

3

Start / End Page

283 / 288

Location

Germany

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Tissue Distribution
  • Neurology & Neurosurgery
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Mass Spectrometry
  • Humans
 

Citation

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Gottfries, J., Mansson, J. E., Fredman, P., Wikstrand, C. J., Friedman, H. S., Bigner, D. D., & Svennerholm, L. (1989). Ganglioside mapping of a human medulloblastoma xenograft. Acta Neuropathol, 77(3), 283–288. https://doi.org/10.1007/BF00687580
Gottfries, J., J. E. Mansson, P. Fredman, C. J. Wikstrand, H. S. Friedman, D. D. Bigner, and L. Svennerholm. “Ganglioside mapping of a human medulloblastoma xenograft.” Acta Neuropathol 77, no. 3 (1989): 283–88. https://doi.org/10.1007/BF00687580.
Gottfries J, Mansson JE, Fredman P, Wikstrand CJ, Friedman HS, Bigner DD, et al. Ganglioside mapping of a human medulloblastoma xenograft. Acta Neuropathol. 1989;77(3):283–8.
Gottfries, J., et al. “Ganglioside mapping of a human medulloblastoma xenograft.” Acta Neuropathol, vol. 77, no. 3, 1989, pp. 283–88. Pubmed, doi:10.1007/BF00687580.
Gottfries J, Mansson JE, Fredman P, Wikstrand CJ, Friedman HS, Bigner DD, Svennerholm L. Ganglioside mapping of a human medulloblastoma xenograft. Acta Neuropathol. 1989;77(3):283–288.
Journal cover image

Published In

Acta Neuropathol

DOI

10.1007/BF00687580

ISSN

0001-6322

Publication Date

1989

Volume

77

Issue

3

Start / End Page

283 / 288

Location

Germany

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Tissue Distribution
  • Neurology & Neurosurgery
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Mass Spectrometry
  • Humans