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Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts.

Publication ,  Journal Article
Bigner, SH; Friedman, HS; Vogelstein, B; Oakes, WJ; Bigner, DD
Published in: Cancer Res
April 15, 1990

Cultured cell lines and xenografts derived from 7 human medulloblastomas were evaluated for amplification of the c-myc, N-myc, epidermal growth factor receptor, and gli genes by Southern blot analysis. Karyotypes of the original biopsies and early passaged cells demonstrated double minute chromosomes in 4 of the 7 cases. All 7 samples (3 cell lines and 4 xenografts) from the 4 tumors with double minute chromosomes contained amplification of the c-myc gene. Cell lines and xenografts derived from the 3 biopsies without double minute chromosomes failed to demonstrate amplification of the 4 genes which were tested, but a rearrangement of the c-myc gene occurred in 1 of the 3 tumors. These observations demonstrate that the c-myc gene is often amplified and/or rearranged in human medulloblastomas and suggest that amplification of this gene provides a growth advantage for medulloblastoma cells in vitro and in vivo.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

April 15, 1990

Volume

50

Issue

8

Start / End Page

2347 / 2350

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Proto-Oncogenes
  • Proto-Oncogene Proteins c-myc
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases
  • Oncology & Carcinogenesis
  • Nucleic Acid Hybridization
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bigner, S. H., Friedman, H. S., Vogelstein, B., Oakes, W. J., & Bigner, D. D. (1990). Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts. Cancer Res, 50(8), 2347–2350.
Bigner, S. H., H. S. Friedman, B. Vogelstein, W. J. Oakes, and D. D. Bigner. “Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts.Cancer Res 50, no. 8 (April 15, 1990): 2347–50.
Bigner SH, Friedman HS, Vogelstein B, Oakes WJ, Bigner DD. Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts. Cancer Res. 1990 Apr 15;50(8):2347–50.
Bigner, S. H., et al. “Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts.Cancer Res, vol. 50, no. 8, Apr. 1990, pp. 2347–50.
Bigner SH, Friedman HS, Vogelstein B, Oakes WJ, Bigner DD. Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts. Cancer Res. 1990 Apr 15;50(8):2347–2350.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

April 15, 1990

Volume

50

Issue

8

Start / End Page

2347 / 2350

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Proto-Oncogenes
  • Proto-Oncogene Proteins c-myc
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases
  • Oncology & Carcinogenesis
  • Nucleic Acid Hybridization
  • Neoplasm Transplantation
  • Mice, Nude
  • Mice