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Sequential adsorption analysis of antibodies to neuroectodermal cells by an indirect quantitative method.

Publication ,  Journal Article
Harwood, SE; Bigner, DD; Wechsler, W; Day, ED
Published in: J Natl Cancer Inst
June 1976

An indirect radioimmunoassay was developed for the sequential adsorption analysis of rabbit antibodies raised against rat neuroectodermal cells. The quantitativee relationship between primary adsorbed rabbit antitumor antibodies and secondary adsorbed goat antibodies to rabbit IgG was explored by paired radioiodine analysis. In the final indirect method, the amount of unlabeled rabbit antibody removed by cultured monolayers of cells at each step in a sequence of cells could be determined from an equation relating the unlabeled amount to values of bound 125I-labeled goat antirabbit IgG. To obtain the total quantity of rabbit antibody in a particular antiserum reagent, a sequential adsorption analysis was done with successive steps of homologous cells. To obtain the amount of antibody that was cross-reactive for other cell lines, we included those lines in the first several steps of the sequence. The sequential adsorption profile was considered as a more important indicator of the quality of a particular antiserum reagent than was the total amount of antibody contained in it. Neuroectodermal cell lines used as illustrative examples included subclones of the C6 astrocytoma and of the RN-2 schwannoma.

Duke Scholars

Published In

J Natl Cancer Inst

DOI

ISSN

0027-8874

Publication Date

June 1976

Volume

56

Issue

6

Start / End Page

1201 / 1205

Location

United States

Related Subject Headings

  • Rats
  • Radioimmunosorbent Test
  • Radioimmunoassay
  • Oncology & Carcinogenesis
  • Neurons
  • Neurilemmoma
  • Neoplasms, Experimental
  • Immunoglobulin G
  • Fibrosarcoma
  • Cross Reactions
 

Citation

APA
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ICMJE
MLA
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Harwood, S. E., Bigner, D. D., Wechsler, W., & Day, E. D. (1976). Sequential adsorption analysis of antibodies to neuroectodermal cells by an indirect quantitative method. J Natl Cancer Inst, 56(6), 1201–1205. https://doi.org/10.1093/jnci/56.6.1201
Harwood, S. E., D. D. Bigner, W. Wechsler, and E. D. Day. “Sequential adsorption analysis of antibodies to neuroectodermal cells by an indirect quantitative method.J Natl Cancer Inst 56, no. 6 (June 1976): 1201–5. https://doi.org/10.1093/jnci/56.6.1201.
Harwood SE, Bigner DD, Wechsler W, Day ED. Sequential adsorption analysis of antibodies to neuroectodermal cells by an indirect quantitative method. J Natl Cancer Inst. 1976 Jun;56(6):1201–5.
Harwood, S. E., et al. “Sequential adsorption analysis of antibodies to neuroectodermal cells by an indirect quantitative method.J Natl Cancer Inst, vol. 56, no. 6, June 1976, pp. 1201–05. Pubmed, doi:10.1093/jnci/56.6.1201.
Harwood SE, Bigner DD, Wechsler W, Day ED. Sequential adsorption analysis of antibodies to neuroectodermal cells by an indirect quantitative method. J Natl Cancer Inst. 1976 Jun;56(6):1201–1205.
Journal cover image

Published In

J Natl Cancer Inst

DOI

ISSN

0027-8874

Publication Date

June 1976

Volume

56

Issue

6

Start / End Page

1201 / 1205

Location

United States

Related Subject Headings

  • Rats
  • Radioimmunosorbent Test
  • Radioimmunoassay
  • Oncology & Carcinogenesis
  • Neurons
  • Neurilemmoma
  • Neoplasms, Experimental
  • Immunoglobulin G
  • Fibrosarcoma
  • Cross Reactions