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A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium.

Publication ,  Journal Article
Fredman, P; Månsson, JE; Wikstrand, CJ; Vrionis, FD; Rynmark, BM; Bigner, DD; Svennerholm, L
Published in: J Biol Chem
July 25, 1989
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A monoclonal antibody produced by immunization with cells of the human glioma cell line D-54 MG reacted with ganglioside GM2. The binding epitope of the antibody was found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal. Immunological detection of glycolipid antigens on thin-layer plates with this monoclonal antibody, DMAb-1, revealed the presence of a new ganglioside. This ganglioside, co-migrating with NeuAc alpha 2-6Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer(6'-LM1) and GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GalNAc beta 1-4Gla beta 1-4Glc beta 1-1Cer (GalNAc-isoGM1) at chromatographic separation was isolated from human meconium. Its structure was determined by permethylation and fast atom bombardment-mass spectometry analyses. The new ganglioside was found to be a combination of the lacto and ganglio series gangliosides, and the structure found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GlcNAc alpha 1-3Gal beta 1-4Glc beta 1-1Cer(GalNAc-3'-isoLM1).

Duke Scholars

Darell Doty Bigner
Author Darell Doty Bigner Neurosurgery

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

July 25, 1989

Volume

264

Issue

21

Start / End Page

12122 / 12125

Location

United States

Related Subject Headings

  • Molecular Sequence Data
  • Meconium
  • Mass Spectrometry
  • Infant, Newborn
  • Humans
  • Glycolipids
  • Gangliosides
  • G(M2) Ganglioside
  • Epitopes
  • Carbohydrate Sequence
 

Citation

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Fredman, P., Månsson, J. E., Wikstrand, C. J., Vrionis, F. D., Rynmark, B. M., Bigner, D. D., & Svennerholm, L. (1989). A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium. J Biol Chem, 264(21), 12122–12125.
Fredman, P., J. E. Månsson, C. J. Wikstrand, F. D. Vrionis, B. M. Rynmark, D. D. Bigner, and L. Svennerholm. “A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium.” J Biol Chem 264, no. 21 (July 25, 1989): 12122–25.
Fredman P, Månsson JE, Wikstrand CJ, Vrionis FD, Rynmark BM, Bigner DD, et al. A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium. J Biol Chem. 1989 Jul 25;264(21):12122–5.
Fredman, P., et al. “A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium.” J Biol Chem, vol. 264, no. 21, July 1989, pp. 12122–25.
Fredman P, Månsson JE, Wikstrand CJ, Vrionis FD, Rynmark BM, Bigner DD, Svennerholm L. A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium. J Biol Chem. 1989 Jul 25;264(21):12122–12125.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

July 25, 1989

Volume

264

Issue

21

Start / End Page

12122 / 12125

Location

United States

Related Subject Headings

  • Molecular Sequence Data
  • Meconium
  • Mass Spectrometry
  • Infant, Newborn
  • Humans
  • Glycolipids
  • Gangliosides
  • G(M2) Ganglioside
  • Epitopes
  • Carbohydrate Sequence