Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification.
Publication
, Journal Article
Wong, AJ; Bigner, SH; Bigner, DD; Kinzler, KW; Hamilton, SR; Vogelstein, B
Published in: Proc Natl Acad Sci U S A
October 1987
Primary malignant gliomas from 63 patients were analyzed to determine the relationship between amplification of the gene encoding the epidermal growth factor receptor (EGFR) and expression of the corresponding mRNA. Twenty-four tumors were found to have amplified the EGFR gene and amplification of other genes occurred in three additional tumors. Hybridization with synthetic RNA probes was used to quantitate mRNA levels in situ. All 24 tumors with amplification of the EGFR gene had high levels of expression of this gene, while none of the 39 tumors without amplification had increased levels. This shows that, in human gliomas, large increases in the expression of the EGFR gene are invariably associated with alterations in gene structure.
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Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
October 1987
Volume
84
Issue
19
Start / End Page
6899 / 6903
Location
United States
Related Subject Headings
- RNA, Messenger
- Oncogenes
- Nucleic Acid Hybridization
- Middle Aged
- Male
- Humans
- Glioma
- Genes
- Gene Amplification
- Female
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Wong, A. J., Bigner, S. H., Bigner, D. D., Kinzler, K. W., Hamilton, S. R., & Vogelstein, B. (1987). Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification. Proc Natl Acad Sci U S A, 84(19), 6899–6903. https://doi.org/10.1073/pnas.84.19.6899
Wong, A. J., S. H. Bigner, D. D. Bigner, K. W. Kinzler, S. R. Hamilton, and B. Vogelstein. “Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification.” Proc Natl Acad Sci U S A 84, no. 19 (October 1987): 6899–6903. https://doi.org/10.1073/pnas.84.19.6899.
Wong AJ, Bigner SH, Bigner DD, Kinzler KW, Hamilton SR, Vogelstein B. Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6899–903.
Wong, A. J., et al. “Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification.” Proc Natl Acad Sci U S A, vol. 84, no. 19, Oct. 1987, pp. 6899–903. Pubmed, doi:10.1073/pnas.84.19.6899.
Wong AJ, Bigner SH, Bigner DD, Kinzler KW, Hamilton SR, Vogelstein B. Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6899–6903.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
October 1987
Volume
84
Issue
19
Start / End Page
6899 / 6903
Location
United States
Related Subject Headings
- RNA, Messenger
- Oncogenes
- Nucleic Acid Hybridization
- Middle Aged
- Male
- Humans
- Glioma
- Genes
- Gene Amplification
- Female