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Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice.

Publication ,  Journal Article
Dawson, HN; Ferreira, A; Eyster, MV; Ghoshal, N; Binder, LI; Vitek, MP
Published in: J Cell Sci
March 2001

Conflicting evidence supports a role for tau as an essential neuronal cytoskeletal protein or as a redundant protein whose function can be fulfilled by other microtubule-associated proteins. To investigate the function of tau in axonogenesis, we created tau deficient mice by disrupting the TAU gene. The engineered mice do not express the tau protein, appear physically normal and are able to reproduce. In contrast to a previously reported tau knockout mouse, embryonic hippocampal cultures from tau deficient mice show a significant delay in maturation as measured by axonal and neuritic extensions. The classic technique of selectively enhancing axonal growth by growth on laminin substrates failed to restore normal neuronal maturation of tau knockout neurons. By mating human TAU-gene transgenic and tau knockout mice, we reconstituted tau-deficient neurons with human tau proteins and restored a normal pattern of axonal growth and neuronal maturation. The ability of human tau proteins to rescue tau-deficient mouse neurons confirms that tau expression affects the rate of neurite extension.

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Published In

J Cell Sci

DOI

ISSN

0021-9533

Publication Date

March 2001

Volume

114

Issue

Pt 6

Start / End Page

1179 / 1187

Location

England

Related Subject Headings

  • tau Proteins
  • Neurons
  • Microtubule-Associated Proteins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Laminin
  • Humans
  • Hippocampus
 

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Dawson, H. N., Ferreira, A., Eyster, M. V., Ghoshal, N., Binder, L. I., & Vitek, M. P. (2001). Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice. J Cell Sci, 114(Pt 6), 1179–1187. https://doi.org/10.1242/jcs.114.6.1179
Dawson, H. N., A. Ferreira, M. V. Eyster, N. Ghoshal, L. I. Binder, and M. P. Vitek. “Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice.J Cell Sci 114, no. Pt 6 (March 2001): 1179–87. https://doi.org/10.1242/jcs.114.6.1179.
Dawson HN, Ferreira A, Eyster MV, Ghoshal N, Binder LI, Vitek MP. Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice. J Cell Sci. 2001 Mar;114(Pt 6):1179–87.
Dawson, H. N., et al. “Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice.J Cell Sci, vol. 114, no. Pt 6, Mar. 2001, pp. 1179–87. Pubmed, doi:10.1242/jcs.114.6.1179.
Dawson HN, Ferreira A, Eyster MV, Ghoshal N, Binder LI, Vitek MP. Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice. J Cell Sci. 2001 Mar;114(Pt 6):1179–1187.
Journal cover image

Published In

J Cell Sci

DOI

ISSN

0021-9533

Publication Date

March 2001

Volume

114

Issue

Pt 6

Start / End Page

1179 / 1187

Location

England

Related Subject Headings

  • tau Proteins
  • Neurons
  • Microtubule-Associated Proteins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Laminin
  • Humans
  • Hippocampus