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Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration.

Publication ,  Journal Article
Lee, Y; Grill, S; Sanchez, A; Murphy-Ryan, M; Poss, KD
Published in: Development
December 2005

During appendage regeneration in urodeles and teleosts, tissue replacement is precisely regulated such that only the appropriate structures are recovered, a phenomenon referred to as positional memory. It is believed that there exists, or is quickly established after amputation, a dynamic gradient of positional information along the proximodistal (PD) axis of the appendage that assigns region-specific instructions to injured tissue. These instructions specify the amount of tissue to regenerate, as well as the rate at which regenerative growth is to occur. A striking theme among many species is that the rate of regeneration is more rapid in proximally amputated appendages compared with distal amputations. However, the underlying molecular regulation is unclear. Here, we identify position-dependent differences in the rate of growth during zebrafish caudal fin regeneration. These growth rates correlate with position-dependent differences in blastemal length, mitotic index and expression of the Fgf target genes mkp3, sef and spry4. To address whether PD differences in amounts of Fgf signaling are responsible for position-dependent blastemal function, we have generated transgenic fish in which Fgf receptor activity can be experimentally manipulated. We find that the level of Fgf signaling exhibits strict control over target gene expression, blastemal proliferation and regenerative growth rate. Our results demonstrate that Fgf signaling defines position-dependent blastemal properties and growth rates for the regenerating zebrafish appendage.

Duke Scholars

Published In

Development

DOI

ISSN

0950-1991

Publication Date

December 2005

Volume

132

Issue

23

Start / End Page

5173 / 5183

Location

England

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Time Factors
  • Signal Transduction
  • Regeneration
  • Protein Tyrosine Phosphatases
  • Nerve Tissue Proteins
  • Membrane Proteins
  • Growth
  • Gene Expression Regulation, Developmental
 

Citation

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Lee, Y., Grill, S., Sanchez, A., Murphy-Ryan, M., & Poss, K. D. (2005). Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration. Development, 132(23), 5173–5183. https://doi.org/10.1242/dev.02101
Lee, Yoonsung, Sara Grill, Angela Sanchez, Maureen Murphy-Ryan, and Kenneth D. Poss. “Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration.Development 132, no. 23 (December 2005): 5173–83. https://doi.org/10.1242/dev.02101.
Lee Y, Grill S, Sanchez A, Murphy-Ryan M, Poss KD. Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration. Development. 2005 Dec;132(23):5173–83.
Lee, Yoonsung, et al. “Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration.Development, vol. 132, no. 23, Dec. 2005, pp. 5173–83. Pubmed, doi:10.1242/dev.02101.
Lee Y, Grill S, Sanchez A, Murphy-Ryan M, Poss KD. Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration. Development. 2005 Dec;132(23):5173–5183.
Journal cover image

Published In

Development

DOI

ISSN

0950-1991

Publication Date

December 2005

Volume

132

Issue

23

Start / End Page

5173 / 5183

Location

England

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Time Factors
  • Signal Transduction
  • Regeneration
  • Protein Tyrosine Phosphatases
  • Nerve Tissue Proteins
  • Membrane Proteins
  • Growth
  • Gene Expression Regulation, Developmental