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Prolonged dark adaptation changes connexin expression in the mouse retina.

Publication ,  Journal Article
Kihara, AH; de Castro, LM; Moriscot, AS; Hamassaki, DE
Published in: J Neurosci Res
May 15, 2006

In the retina, ambient light levels influence the cell coupling provided by gap junction (GJ) channels, to compensate the visual function for various lighting conditions. However, the effects of ambient light levels on expression of connexins (Cx), the proteins that form the GJ channels, are poorly understood. In the present study, we first determined whether gene expression of specific Cx (Cx26, Cx31.1, Cx36, Cx37, Cx40, Cx43, Cx45, Cx50, and Cx57) was affected by prolonged dark adaptation. Cx mRNA relative levels were determined in mouse retinas dark adapted for 3 hr, 1 day, and 7 days by using quantitative real-time PCR. Transcript levels of some Cx were repressed after 3 hr (Cx57), 1 day (Cx45), or 7 days (Cx36 and Cx43) of dark adaptation; others were increased after 1 day (Cx50) or 7 days (Cx31.1 and Cx37); and two of them (Cx26 and Cx40) were not significantly altered. The second aim was to determine whether prolonged dark adaptation affects protein expression of two important Cx in retina: neuronal Cx36 and glial Cx43. We were able to demonstrate that important changes in protein distribution and expression also took place in retina during long-term dark adaptation. Given their localization, the specific alterations in Cx expression may reflect their distinct response to ambient light levels.

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Published In

J Neurosci Res

DOI

ISSN

0360-4012

Publication Date

May 15, 2006

Volume

83

Issue

7

Start / End Page

1331 / 1341

Location

United States

Related Subject Headings

  • Vision, Ocular
  • Retina
  • Photic Stimulation
  • Neurons
  • Neurology & Neurosurgery
  • Neuroglia
  • Mice, Inbred C57BL
  • Mice
  • Light
  • Gene Expression Regulation
 

Citation

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Kihara, A. H., de Castro, L. M., Moriscot, A. S., & Hamassaki, D. E. (2006). Prolonged dark adaptation changes connexin expression in the mouse retina. J Neurosci Res, 83(7), 1331–1341. https://doi.org/10.1002/jnr.20815
Kihara, Alexandre Hiroaki, Leandro Mantovani de Castro, Anselmo Sigari Moriscot, and Dânia Emi Hamassaki. “Prolonged dark adaptation changes connexin expression in the mouse retina.J Neurosci Res 83, no. 7 (May 15, 2006): 1331–41. https://doi.org/10.1002/jnr.20815.
Kihara AH, de Castro LM, Moriscot AS, Hamassaki DE. Prolonged dark adaptation changes connexin expression in the mouse retina. J Neurosci Res. 2006 May 15;83(7):1331–41.
Kihara, Alexandre Hiroaki, et al. “Prolonged dark adaptation changes connexin expression in the mouse retina.J Neurosci Res, vol. 83, no. 7, May 2006, pp. 1331–41. Pubmed, doi:10.1002/jnr.20815.
Kihara AH, de Castro LM, Moriscot AS, Hamassaki DE. Prolonged dark adaptation changes connexin expression in the mouse retina. J Neurosci Res. 2006 May 15;83(7):1331–1341.
Journal cover image

Published In

J Neurosci Res

DOI

ISSN

0360-4012

Publication Date

May 15, 2006

Volume

83

Issue

7

Start / End Page

1331 / 1341

Location

United States

Related Subject Headings

  • Vision, Ocular
  • Retina
  • Photic Stimulation
  • Neurons
  • Neurology & Neurosurgery
  • Neuroglia
  • Mice, Inbred C57BL
  • Mice
  • Light
  • Gene Expression Regulation