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Molecular cloning and characterization of Aspergillus nidulans cyclophilin B.

Publication ,  Journal Article
Joseph, JD; Heitman, J; Means, AR
Published in: Fungal Genet Biol
June 1999

Cyclophilins are an evolutionarily conserved family of proteins which serve as the intracellular receptors for the immunosuppressive drug cyclosporin A. Here we report the characterization of the first cyclophilin cloned from the filamentous fungus Aspergillus nidulans (CYPB). Sequence analysis of the cypB gene predicts an encoded protein with highest homology to the murine cyclophilin B protein. The sequence similarity includes an N-terminal sequence predicted to target the protein to the endoplasmic reticulum (ER) as well as a C-terminal sequence predicted to retain the mature protein in the ER. The bacterially expressed hexa-histidine tagged protein displays peptidyl-prolyl isomerase activity which is inhibited by cyclosporin A. In the presence of cyclosporin A, the expressed protein also inhibits purified calcineurin. When the endogenous cypB gene was disrupted and placed under the control of the regulatable alcohol dehydrogenase promoter, the strain demonstrated no detectable growth phenotype under conditions which induce or repress cypB transcription. Induction or repression of the cypB gene also did not effect sensitivity of A. nidulans to cyclosporin A. cypB mRNA levels were significantly elevated under severe heat shock conditions, indicating a possible role for the A. nidulans cyclophilin B protein during growth in high stress environments.

Duke Scholars

Published In

Fungal Genet Biol

DOI

ISSN

1087-1845

Publication Date

June 1999

Volume

27

Issue

1

Start / End Page

55 / 66

Location

United States

Related Subject Headings

  • Threonine
  • Sequence Homology, Amino Acid
  • Recombinant Fusion Proteins
  • RNA, Messenger
  • Peptidylprolyl Isomerase
  • Molecular Sequence Data
  • Microbiology
  • Microbial Sensitivity Tests
  • Immunophilins
  • Hot Temperature
 

Citation

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ICMJE
MLA
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Joseph, J. D., Heitman, J., & Means, A. R. (1999). Molecular cloning and characterization of Aspergillus nidulans cyclophilin B. Fungal Genet Biol, 27(1), 55–66. https://doi.org/10.1006/fgbi.1999.1131
Joseph, J. D., J. Heitman, and A. R. Means. “Molecular cloning and characterization of Aspergillus nidulans cyclophilin B.Fungal Genet Biol 27, no. 1 (June 1999): 55–66. https://doi.org/10.1006/fgbi.1999.1131.
Joseph JD, Heitman J, Means AR. Molecular cloning and characterization of Aspergillus nidulans cyclophilin B. Fungal Genet Biol. 1999 Jun;27(1):55–66.
Joseph, J. D., et al. “Molecular cloning and characterization of Aspergillus nidulans cyclophilin B.Fungal Genet Biol, vol. 27, no. 1, June 1999, pp. 55–66. Pubmed, doi:10.1006/fgbi.1999.1131.
Joseph JD, Heitman J, Means AR. Molecular cloning and characterization of Aspergillus nidulans cyclophilin B. Fungal Genet Biol. 1999 Jun;27(1):55–66.
Journal cover image

Published In

Fungal Genet Biol

DOI

ISSN

1087-1845

Publication Date

June 1999

Volume

27

Issue

1

Start / End Page

55 / 66

Location

United States

Related Subject Headings

  • Threonine
  • Sequence Homology, Amino Acid
  • Recombinant Fusion Proteins
  • RNA, Messenger
  • Peptidylprolyl Isomerase
  • Molecular Sequence Data
  • Microbiology
  • Microbial Sensitivity Tests
  • Immunophilins
  • Hot Temperature