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Interactions of calmodulin with coated vesicles from brain.

Publication ,  Journal Article
Linden, CD; Dedman, JR; Chafouleas, JG; Means, AR; Roth, TF
Published in: Proc Natl Acad Sci U S A
January 1981
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Coated vesicles purified in the presence of calcium are enriched approximately 7-fold in calmodulin content relative to standard preparations isolated in the absence of free calcium. Radioiodinated calmodulin binds specifically to coated vesicles in vitro. Binding is saturable (Kd, 10 nM) and calcium dependent. Half-maximal binding occurs at 2.4 microM free Ca2+ whereas up to 1.2 mM Mg2+ has no effect on binding. Troponin C, a protein homologous to calmodulin, competes with binding of 125I-labeled calmodulin with 1/30th the affinity of native calmodulin. Chromatography of 2 M urea-solubilized coated vesicles on a calmodulin-Sepharose column demonstrated a Ca2+-dependent interaction of coated vesicle proteins and calmodulin. The properties of calmodulin binding to coated vesicles are comparable to those of calmodulin activities in other systems.

Duke Scholars

Anthony Ross Means
Author Anthony Ross Means Pharmacology & Cancer Biology

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.78.1.308

ISSN

0027-8424

Publication Date

January 1981

Volume

78

Issue

1

Start / End Page

308 / 312

Location

United States

Related Subject Headings

  • Troponin C
  • Troponin
  • Radioimmunoassay
  • Chromatography, Affinity
  • Calmodulin
  • Calcium-Binding Proteins
  • Calcium
  • Brain
  • Binding, Competitive
  • Animals
 

Citation

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Linden, C. D., Dedman, J. R., Chafouleas, J. G., Means, A. R., & Roth, T. F. (1981). Interactions of calmodulin with coated vesicles from brain. Proc Natl Acad Sci U S A, 78(1), 308–312. https://doi.org/10.1073/pnas.78.1.308
Linden, C. D., J. R. Dedman, J. G. Chafouleas, A. R. Means, and T. F. Roth. “Interactions of calmodulin with coated vesicles from brain.” Proc Natl Acad Sci U S A 78, no. 1 (January 1981): 308–12. https://doi.org/10.1073/pnas.78.1.308.
Linden CD, Dedman JR, Chafouleas JG, Means AR, Roth TF. Interactions of calmodulin with coated vesicles from brain. Proc Natl Acad Sci U S A. 1981 Jan;78(1):308–12.
Linden, C. D., et al. “Interactions of calmodulin with coated vesicles from brain.” Proc Natl Acad Sci U S A, vol. 78, no. 1, Jan. 1981, pp. 308–12. Pubmed, doi:10.1073/pnas.78.1.308.
Linden CD, Dedman JR, Chafouleas JG, Means AR, Roth TF. Interactions of calmodulin with coated vesicles from brain. Proc Natl Acad Sci U S A. 1981 Jan;78(1):308–312.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.78.1.308

ISSN

0027-8424

Publication Date

January 1981

Volume

78

Issue

1

Start / End Page

308 / 312

Location

United States

Related Subject Headings

  • Troponin C
  • Troponin
  • Radioimmunoassay
  • Chromatography, Affinity
  • Calmodulin
  • Calcium-Binding Proteins
  • Calcium
  • Brain
  • Binding, Competitive
  • Animals