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Sequence homology of the Ca2+-dependent regulator of cyclic nucleotide phosphodiesterase from rat testis with other Ca2+-binding proteins.

Publication ,  Journal Article
Dedman, JR; Jackson, RL; Schreiber, WE; Means, AR
Published in: J Biol Chem
January 25, 1978

A Ca2+-dependent regulator protein of cyclic 3':5'-nucleotide phosphodiesterase (EC 3.1.4.17) has previously been isolated from rat testis and shown to be a heat-stable, Ca2+-binding protein with a molecular weight of approximately 17,000. The Ca2+-dependent regulator protein is also structurally similar to troponin-C, the Ca2+-binding component of muscle troponin and Ca2+ mediator of muscle contraction. The present report describes a partial amino acid sequence of the Ca2+-dependent regulator. The protein (148 amino acids) is 50% homologous with skeletal muscle troponin-C, but is 11 residues shorter than the muscle protein. The Ca2+-dependent regulator protein has an NH2-terminal sequence of acetyl-Ala-Asp-Glu, a COOH-terminal sequence of Thr-Ala-Lys and 1 residue of epsilon-trimethyllysine located at position 115. All of these properties are distinct from those of other homologous Ca2+-binding proteins. These properties may account for the biological specificities demonstrated by these proteins as compared to the Ca2+-dependent regulator protein. Based on the sequence and a comparison of the Ca2+-dependent regulator protein to other calcium-binding proteins, our data support the view that all of these moecules contain common sequences, especially at their proposed metal-binding sites.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

January 25, 1978

Volume

253

Issue

2

Start / End Page

343 / 346

Location

United States

Related Subject Headings

  • Troponin
  • Testis
  • Rats
  • Peptide Fragments
  • Molecular Weight
  • Male
  • Carrier Proteins
  • Calcium
  • Biochemistry & Molecular Biology
  • Animals
 

Citation

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Dedman, J. R., Jackson, R. L., Schreiber, W. E., & Means, A. R. (1978). Sequence homology of the Ca2+-dependent regulator of cyclic nucleotide phosphodiesterase from rat testis with other Ca2+-binding proteins. J Biol Chem, 253(2), 343–346.
Dedman, J. R., R. L. Jackson, W. E. Schreiber, and A. R. Means. “Sequence homology of the Ca2+-dependent regulator of cyclic nucleotide phosphodiesterase from rat testis with other Ca2+-binding proteins.J Biol Chem 253, no. 2 (January 25, 1978): 343–46.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

January 25, 1978

Volume

253

Issue

2

Start / End Page

343 / 346

Location

United States

Related Subject Headings

  • Troponin
  • Testis
  • Rats
  • Peptide Fragments
  • Molecular Weight
  • Male
  • Carrier Proteins
  • Calcium
  • Biochemistry & Molecular Biology
  • Animals