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Flagellar motility requires the cAMP-dependent phosphorylation of a heat-stable NP-40-soluble 56 kd protein, axokinin.

Publication ,  Journal Article
Tash, JS; Kakar, SS; Means, AR
Published in: Cell
September 1984

Using NP-40-treated dog sperm as a model, the stimulatory effect of cAMP upon reactivated flagellar motility has been shown to be dependent upon the cAMP-dependent phosphorylation of a heat-stable NP-40-soluble protein of 56 kd. Examination by two-dimensional polyacrylamide gel electrophoresis of NP-40 extract proteins phosphorylated with gamma-32P-ATP revealed a major cAMP-dependent phosphopeptide at 56 kd. This is the only cAMP-dependent phosphoprotein common to NP-40 extracts of all tissues that show cAMP-dependent stimulation of flagellar motility. These cells and tissues include sea urchin, dog, and human sperm, as well as dog trachea and retina. Moreover, this phosphoprotein is absent in nonstimulatory extracts from tissues such as skeletal muscle, brain, and liver. We conclude that the cAMP-dependent phosphorylation of the 56 kd peptide represents a major regulatory component of not only sperm but other types of axonemal motility as well.

Duke Scholars

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

September 1984

Volume

38

Issue

2

Start / End Page

551 / 559

Location

United States

Related Subject Headings

  • Spermatozoa
  • Sperm Tail
  • Sperm Motility
  • Sea Urchins
  • Polyethylene Glycols
  • Phosphorylation
  • Phosphoproteins
  • Octoxynol
  • Male
  • Humans
 

Citation

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Tash, J. S., Kakar, S. S., & Means, A. R. (1984). Flagellar motility requires the cAMP-dependent phosphorylation of a heat-stable NP-40-soluble 56 kd protein, axokinin. Cell, 38(2), 551–559. https://doi.org/10.1016/0092-8674(84)90509-9
Tash, J. S., S. S. Kakar, and A. R. Means. “Flagellar motility requires the cAMP-dependent phosphorylation of a heat-stable NP-40-soluble 56 kd protein, axokinin.Cell 38, no. 2 (September 1984): 551–59. https://doi.org/10.1016/0092-8674(84)90509-9.
Tash, J. S., et al. “Flagellar motility requires the cAMP-dependent phosphorylation of a heat-stable NP-40-soluble 56 kd protein, axokinin.Cell, vol. 38, no. 2, Sept. 1984, pp. 551–59. Pubmed, doi:10.1016/0092-8674(84)90509-9.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

September 1984

Volume

38

Issue

2

Start / End Page

551 / 559

Location

United States

Related Subject Headings

  • Spermatozoa
  • Sperm Tail
  • Sperm Motility
  • Sea Urchins
  • Polyethylene Glycols
  • Phosphorylation
  • Phosphoproteins
  • Octoxynol
  • Male
  • Humans