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Characterization and analysis of an apparent autophosphorylation of chicken gizzard myosin light chain kinase.

Publication ,  Journal Article
Foyt, HL; Means, AR
Published in: J Cyclic Nucleotide Protein Phosphor Res
1985

A phosphorylation occurs at two sites in chicken gizzard myosin light chain kinase that appears to be catalyzed by an autophosphorylation reaction. This reaction is inhibited by approximately 75% in the presence of Ca2+-calmodulin, but is unaffected by the cAMP-dependent protein kinase inhibitor. Whereas the catalytic subunit of cAMP-dependent protein kinase phosphorylates myosin light chain kinase at only serine residues, the non cAMP-dependent phosphorylation occurs at both serine and threonine residues. One, if not both, of these latter sites are distinct from the sites recognized by the catalytic subunit of cAMP-dependent protein kinase. Consequently, there must be at least three and possibly four sites in myosin light chain kinase capable of incorporating phosphate, either in response to catalytic subunit or by autophosphorylation.

Duke Scholars

Published In

J Cyclic Nucleotide Protein Phosphor Res

ISSN

0746-3898

Publication Date

1985

Volume

10

Issue

2

Start / End Page

143 / 155

Location

United States

Related Subject Headings

  • Protein Kinases
  • Phosphorylation
  • Phosphates
  • Myosin-Light-Chain Kinase
  • In Vitro Techniques
  • Gizzard, Avian
  • Electrophoresis, Polyacrylamide Gel
  • Chickens
  • Animals
 

Citation

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Foyt, H. L., & Means, A. R. (1985). Characterization and analysis of an apparent autophosphorylation of chicken gizzard myosin light chain kinase. J Cyclic Nucleotide Protein Phosphor Res, 10(2), 143–155.
Foyt, H. L., and A. R. Means. “Characterization and analysis of an apparent autophosphorylation of chicken gizzard myosin light chain kinase.J Cyclic Nucleotide Protein Phosphor Res 10, no. 2 (1985): 143–55.
Foyt HL, Means AR. Characterization and analysis of an apparent autophosphorylation of chicken gizzard myosin light chain kinase. J Cyclic Nucleotide Protein Phosphor Res. 1985;10(2):143–55.
Foyt, H. L., and A. R. Means. “Characterization and analysis of an apparent autophosphorylation of chicken gizzard myosin light chain kinase.J Cyclic Nucleotide Protein Phosphor Res, vol. 10, no. 2, 1985, pp. 143–55.
Foyt HL, Means AR. Characterization and analysis of an apparent autophosphorylation of chicken gizzard myosin light chain kinase. J Cyclic Nucleotide Protein Phosphor Res. 1985;10(2):143–155.

Published In

J Cyclic Nucleotide Protein Phosphor Res

ISSN

0746-3898

Publication Date

1985

Volume

10

Issue

2

Start / End Page

143 / 155

Location

United States

Related Subject Headings

  • Protein Kinases
  • Phosphorylation
  • Phosphates
  • Myosin-Light-Chain Kinase
  • In Vitro Techniques
  • Gizzard, Avian
  • Electrophoresis, Polyacrylamide Gel
  • Chickens
  • Animals