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Estrogen induction of ovalbumin mRNA: evidence for transcription control.

Publication ,  Journal Article
Means, AR; Woo, S; Harris, SE; O'Malley, BW
Published in: Mol Cell Biochem
April 30, 1975

Estrogen induces the synthesis and accumulation of the specific messenger RNA for the egg white protein ovalbumin. The messenger RNA has been purified to apparent homogeneity on a preparative scale and utilized to synthesize a radioactive complementary DNA copy. This complementary DNA probe was first used to reveal that although ovalbumin constitutes 60% of the total protein of chick oviduct the gene which codes for the ovalbumin nRNA is represented only once in each haploid genome: The induction of genetranscription and subsequent accumulation of ovalbumin mRNA during estrogen-mediated tissue differentiation was also investigated. Ovalbumin mRNA sequences were quantifiedusing the complementary DNA probe and by an in vitro heterologous translation system. Similar experiments were performed using chicks which were withdrawn from hormone treatment and then given a single injection of estrogen. The data suggest pure transcriptional control for the mechanism by which estrogen regulates the synthesis of the tissuespecific protein ovalbumin. Finally, several in vitro translation systems are comparedwith respect to their usefullness to assess the effects of hormones on mRNA production. It is concluded that the protein synthesis system derived from wheat germ offers thegreatest advantages for initial studies.

Duke Scholars

Published In

Mol Cell Biochem

DOI

ISSN

0300-8177

Publication Date

April 30, 1975

Volume

7

Issue

1

Start / End Page

33 / 42

Location

Netherlands

Related Subject Headings

  • Triticum
  • Transcription, Genetic
  • Time Factors
  • RNA, Messenger
  • Plants
  • Oviducts
  • Ovalbumin
  • Female
  • Estrogens
  • Diethylstilbestrol
 

Citation

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Means, A. R., Woo, S., Harris, S. E., & O’Malley, B. W. (1975). Estrogen induction of ovalbumin mRNA: evidence for transcription control. Mol Cell Biochem, 7(1), 33–42. https://doi.org/10.1007/BF01732161
Means, A. R., S. Woo, S. E. Harris, and B. W. O’Malley. “Estrogen induction of ovalbumin mRNA: evidence for transcription control.Mol Cell Biochem 7, no. 1 (April 30, 1975): 33–42. https://doi.org/10.1007/BF01732161.
Means AR, Woo S, Harris SE, O’Malley BW. Estrogen induction of ovalbumin mRNA: evidence for transcription control. Mol Cell Biochem. 1975 Apr 30;7(1):33–42.
Means, A. R., et al. “Estrogen induction of ovalbumin mRNA: evidence for transcription control.Mol Cell Biochem, vol. 7, no. 1, Apr. 1975, pp. 33–42. Pubmed, doi:10.1007/BF01732161.
Means AR, Woo S, Harris SE, O’Malley BW. Estrogen induction of ovalbumin mRNA: evidence for transcription control. Mol Cell Biochem. 1975 Apr 30;7(1):33–42.
Journal cover image

Published In

Mol Cell Biochem

DOI

ISSN

0300-8177

Publication Date

April 30, 1975

Volume

7

Issue

1

Start / End Page

33 / 42

Location

Netherlands

Related Subject Headings

  • Triticum
  • Transcription, Genetic
  • Time Factors
  • RNA, Messenger
  • Plants
  • Oviducts
  • Ovalbumin
  • Female
  • Estrogens
  • Diethylstilbestrol