The calmodulin-dependent protein phosphatase catalytic subunit (calcineurin A) is an essential gene in Aspergillus nidulans.
The gene encoding the homologue of the catalytic subunit of the Ca2+/calmodulin-regulated protein phosphatase 2B (calcineurin A) has been isolated from Aspergillus nidulans. This gene, cnaA+, is essential in this fungal system. Analysis of growth-arrested cells following gene disruption by homologous recombination reveals that they are blocked early in the cell cycle. The cnaA+ gene encodes a 2.5 kb mRNA and the deduced protein sequence is highly homologous to the calcineurin A subunit of other species. The mRNA varies in a cell cycle-dependent manner with maximal levels found early in G1 and considerably before the G1/S boundary. As calmodulin is also essential for A.nidulans cell cycle progression and levels rise before the G1/S boundary, our data suggest that calcineurin may represent a primary target for calmodulin at this cell cycle transition point.
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- Sequence Analysis, DNA
- Sequence Alignment
- RNA, Messenger
- RNA, Fungal
- Phosphoprotein Phosphatases
- Mutagenesis, Insertional
- Molecular Sequence Data
- Genes, Fungal
- Gene Expression Regulation, Fungal
- Developmental Biology
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Sequence Analysis, DNA
- Sequence Alignment
- RNA, Messenger
- RNA, Fungal
- Phosphoprotein Phosphatases
- Mutagenesis, Insertional
- Molecular Sequence Data
- Genes, Fungal
- Gene Expression Regulation, Fungal
- Developmental Biology