Skip to main content

The presence of parvalbumin in a nonmuscle cell line attenuates progression through mitosis.

Publication ,  Journal Article
Rasmussen, CD; Means, AR
Published in: Mol Endocrinol
March 1989

Based on studies that have examined the effect of calcium chelators on cells, it has been proposed that this cation plays a role in regulating cell proliferation. In this study a novel approach was used to indirectly examine the role of calcium in cell cycle progression. A cDNA for the Ca2+-binding protein parvalbumin has been expressed in mouse C127 cells, using a bovine papilloma virus-based expression vector. The normal role of parvalbumin is that of a calcium buffer in vertebrate fast twitch muscle, and the C127 cells do not normally express this protein. The presence of parvalbumin had several effects on the growth of C127 cells. The most striking phenotype was an increase in cell cycle duration which analysis showed was the result of an increase the length of G1 and mitosis (predominantly at prophase). Since changes in cell cycle duration typically occur as a result of changes in G1 duration, the observed increase in the length of mitosis is most unusual. The present results indicate that the previously observed increase in the rate of cell proliferation in cells with elevated calmodulin levels is not the result of a general increase in the level of cytoplasmic calcium-binding protein, but is specific to calmodulin. In addition, the results suggest that calcium regulates progression through mitosis by both calmodulin-dependent (metaphase transition) and -independent (prophase) mechanisms.

Duke Scholars

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

March 1989

Volume

3

Issue

3

Start / End Page

588 / 596

Location

United States

Related Subject Headings

  • RNA, Messenger
  • Parvalbumins
  • Muscle Proteins
  • Mitosis
  • Mice
  • Genetic Vectors
  • Genetic Engineering
  • Endocrinology & Metabolism
  • DNA Probes
  • Cell Line
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Rasmussen, C. D., & Means, A. R. (1989). The presence of parvalbumin in a nonmuscle cell line attenuates progression through mitosis. Mol Endocrinol, 3(3), 588–596. https://doi.org/10.1210/mend-3-3-588
Rasmussen, C. D., and A. R. Means. “The presence of parvalbumin in a nonmuscle cell line attenuates progression through mitosis.Mol Endocrinol 3, no. 3 (March 1989): 588–96. https://doi.org/10.1210/mend-3-3-588.
Rasmussen CD, Means AR. The presence of parvalbumin in a nonmuscle cell line attenuates progression through mitosis. Mol Endocrinol. 1989 Mar;3(3):588–96.
Rasmussen, C. D., and A. R. Means. “The presence of parvalbumin in a nonmuscle cell line attenuates progression through mitosis.Mol Endocrinol, vol. 3, no. 3, Mar. 1989, pp. 588–96. Pubmed, doi:10.1210/mend-3-3-588.
Rasmussen CD, Means AR. The presence of parvalbumin in a nonmuscle cell line attenuates progression through mitosis. Mol Endocrinol. 1989 Mar;3(3):588–596.

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

March 1989

Volume

3

Issue

3

Start / End Page

588 / 596

Location

United States

Related Subject Headings

  • RNA, Messenger
  • Parvalbumins
  • Muscle Proteins
  • Mitosis
  • Mice
  • Genetic Vectors
  • Genetic Engineering
  • Endocrinology & Metabolism
  • DNA Probes
  • Cell Line