Skip to main content

BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells.

Publication ,  Journal Article
Vaughn, JP; Davis, PL; Jarboe, MD; Huper, G; Evans, AC; Wiseman, RW; Berchuck, A; Iglehart, JD; Futreal, PA; Marks, JR
Published in: Cell Growth Differ
June 1996

Insight into the function of the BRCA1 tumor suppressor gene may be gained by studying its regulation. In this study, the expression of BRCA1 was examined as a function of the cell cycle in normal and tumor-derived breast epithelial cells. Cells arrested in G(zero) or early in G1 contained low levels of BRCA1 mRNA. After release, populations of cells reached maximal levels of BRCA1 in late G1 and S phase. Induction of BRCA1 was shown to occur before the onset of DNA synthesis by synchronizing cells at the G1-S boundary. Levels of the BRCA1 protein were regulated in a similar manner. No difference was observed between primary cultures of normal mammary epithelial cells and immortalized tumor-derived cell lines. These results suggest that BRCA1 may function at the G1-S checkpoint.

Duke Scholars

Published In

Cell Growth Differ

ISSN

1044-9523

Publication Date

June 1996

Volume

7

Issue

6

Start / End Page

711 / 715

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Reference Values
  • Point Mutation
  • Ovary
  • Oncology & Carcinogenesis
  • Humans
  • Genes, BRCA1
  • Gene Expression Regulation, Neoplastic
  • Gene Expression Regulation
  • G1 Phase
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Vaughn, J. P., Davis, P. L., Jarboe, M. D., Huper, G., Evans, A. C., Wiseman, R. W., … Marks, J. R. (1996). BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells. Cell Growth Differ, 7(6), 711–715.
Vaughn, J. P., P. L. Davis, M. D. Jarboe, G. Huper, A. C. Evans, R. W. Wiseman, A. Berchuck, J. D. Iglehart, P. A. Futreal, and J. R. Marks. “BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells.Cell Growth Differ 7, no. 6 (June 1996): 711–15.
Vaughn JP, Davis PL, Jarboe MD, Huper G, Evans AC, Wiseman RW, et al. BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells. Cell Growth Differ. 1996 Jun;7(6):711–5.
Vaughn, J. P., et al. “BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells.Cell Growth Differ, vol. 7, no. 6, June 1996, pp. 711–15.
Vaughn JP, Davis PL, Jarboe MD, Huper G, Evans AC, Wiseman RW, Berchuck A, Iglehart JD, Futreal PA, Marks JR. BRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells. Cell Growth Differ. 1996 Jun;7(6):711–715.

Published In

Cell Growth Differ

ISSN

1044-9523

Publication Date

June 1996

Volume

7

Issue

6

Start / End Page

711 / 715

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Reference Values
  • Point Mutation
  • Ovary
  • Oncology & Carcinogenesis
  • Humans
  • Genes, BRCA1
  • Gene Expression Regulation, Neoplastic
  • Gene Expression Regulation
  • G1 Phase