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Reactivity of epidermal growth factor receptor monoclonal antibodies with human uterine tissues.

Publication ,  Journal Article
Berchuck, A; Soisson, AP; Soper, JT; Clarke-Pearson, DL; Bast, RC; McCarty, KS
Published in: Arch Pathol Lab Med
October 1989

Two monoclonal antibodies (29.1 and 528), which were raised against the epidermal growth factor receptor, were used to evaluate expression of epidermal growth factor receptor in frozen uterine tissue with immunohistochemical techniques. Monoclonal antibody 29.1 stained only vascular endothelium and glandular epithelium in patients who were blood type A. This staining pattern is consistent with the previously reported blood type A specificity of this antibody. Monoclonal antibody 528, which recognizes a peptide determinant is thought to be specific for the epidermal growth factor receptor, stained both endometrial glands and endometrial stromal cells heavily. Faint staining was also seen in myometrium in most cases. This marked difference in expression of epidermal growth factor receptor between endometrium and myometrium contrasts with results of prior biochemical studies in which tissue homogenates were used. No variation in intensity of staining was seen between proliferative and secretory endometrium with the use of either antibody.

Duke Scholars

Published In

Arch Pathol Lab Med

ISSN

0003-9985

Publication Date

October 1989

Volume

113

Issue

10

Start / End Page

1155 / 1158

Location

United States

Related Subject Headings

  • Uterus
  • Pathology
  • Myometrium
  • Menstrual Cycle
  • Immunohistochemistry
  • Humans
  • Female
  • ErbB Receptors
  • Endometrium
  • Antibodies, Monoclonal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Berchuck, A., Soisson, A. P., Soper, J. T., Clarke-Pearson, D. L., Bast, R. C., & McCarty, K. S. (1989). Reactivity of epidermal growth factor receptor monoclonal antibodies with human uterine tissues. Arch Pathol Lab Med, 113(10), 1155–1158.
Berchuck, A., A. P. Soisson, J. T. Soper, D. L. Clarke-Pearson, R. C. Bast, and K. S. McCarty. “Reactivity of epidermal growth factor receptor monoclonal antibodies with human uterine tissues.Arch Pathol Lab Med 113, no. 10 (October 1989): 1155–58.
Berchuck A, Soisson AP, Soper JT, Clarke-Pearson DL, Bast RC, McCarty KS. Reactivity of epidermal growth factor receptor monoclonal antibodies with human uterine tissues. Arch Pathol Lab Med. 1989 Oct;113(10):1155–8.
Berchuck, A., et al. “Reactivity of epidermal growth factor receptor monoclonal antibodies with human uterine tissues.Arch Pathol Lab Med, vol. 113, no. 10, Oct. 1989, pp. 1155–58.
Berchuck A, Soisson AP, Soper JT, Clarke-Pearson DL, Bast RC, McCarty KS. Reactivity of epidermal growth factor receptor monoclonal antibodies with human uterine tissues. Arch Pathol Lab Med. 1989 Oct;113(10):1155–1158.

Published In

Arch Pathol Lab Med

ISSN

0003-9985

Publication Date

October 1989

Volume

113

Issue

10

Start / End Page

1155 / 1158

Location

United States

Related Subject Headings

  • Uterus
  • Pathology
  • Myometrium
  • Menstrual Cycle
  • Immunohistochemistry
  • Humans
  • Female
  • ErbB Receptors
  • Endometrium
  • Antibodies, Monoclonal