Skip to main content
Journal cover image

Axotomized mouse retinal ganglion cells containing melanopsin show enhanced survival, but not enhanced axon regrowth into a peripheral nerve graft.

Publication ,  Journal Article
Robinson, GA; Madison, RD
Published in: Vision Res
October 2004

Melanopsin is found in only approximately 2% of mouse retinal ganglion cells (RGCs), making these RGCs uniquely and directly photosensitive. Given that the majority of RGCs die after axotomy and that grafting of a peripheral nerve to the eye provides a permissive environment for axon regrowth, the present study examined the survival and axonal regrowth of melanopsin-containing RGCs in mice. One month after optic nerve transection and grafting, RGCs with regrown axons were labeled from the grafts and retinae were processed to visualize melanopsin and TUJ1. Melanopsin-positive and negative RGCs were counted and compared to axotomized RGCs from ungrafted eyes and uninjured RGCs. Melanopsin-positive RGCs showed a 3-fold increase in survival rate compared to non-melanopsin RGCs. Despite this enhanced survival, melanopsin-containing RGCs did not show increased axon regrowth into nerve grafts.

Duke Scholars

Published In

Vision Res

DOI

ISSN

0042-6989

Publication Date

October 2004

Volume

44

Issue

23

Start / End Page

2667 / 2674

Location

England

Related Subject Headings

  • Rod Opsins
  • Retinal Ganglion Cells
  • Peripheral Nerves
  • Nerve Regeneration
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Immunoenzyme Techniques
  • Female
  • Experimental Psychology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Robinson, G. A., & Madison, R. D. (2004). Axotomized mouse retinal ganglion cells containing melanopsin show enhanced survival, but not enhanced axon regrowth into a peripheral nerve graft. Vision Res, 44(23), 2667–2674. https://doi.org/10.1016/j.visres.2004.06.010
Robinson, G. A., and R. D. Madison. “Axotomized mouse retinal ganglion cells containing melanopsin show enhanced survival, but not enhanced axon regrowth into a peripheral nerve graft.Vision Res 44, no. 23 (October 2004): 2667–74. https://doi.org/10.1016/j.visres.2004.06.010.
Robinson, G. A., and R. D. Madison. “Axotomized mouse retinal ganglion cells containing melanopsin show enhanced survival, but not enhanced axon regrowth into a peripheral nerve graft.Vision Res, vol. 44, no. 23, Oct. 2004, pp. 2667–74. Pubmed, doi:10.1016/j.visres.2004.06.010.
Journal cover image

Published In

Vision Res

DOI

ISSN

0042-6989

Publication Date

October 2004

Volume

44

Issue

23

Start / End Page

2667 / 2674

Location

England

Related Subject Headings

  • Rod Opsins
  • Retinal Ganglion Cells
  • Peripheral Nerves
  • Nerve Regeneration
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Immunoenzyme Techniques
  • Female
  • Experimental Psychology