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Potentiation of vagal contractile response by thromboxane mimetic U-46619.

Publication ,  Journal Article
Munoz, NM; Shioya, T; Murphy, TM; Primack, S; Dame, C; Sands, MF; Leff, AR
Published in: J Appl Physiol (1985)
September 1986

We studied the effect of the thromboxane mimetic U-46619 on tracheal smooth muscle contraction caused by bilateral stimulation of the vagus nerves in 14 mongrel dogs in situ. The parasympathetic contractile response was studied isometrically after beta-adrenergic blockade with 2 mg/kg iv propranolol plus 20 micrograms X kg-1 X min-1 continuous intravenous infusion and blockade of endogenous prostaglandin synthesis with 5 mg/kg iv indomethacin. An initial frequency-response curve was generated by electrical stimulation of the caudal ends of cut cervical vagi over the range of frequencies 2-25 Hz (constant 25 V) at 15-s intervals. In five dogs, 10(-10) to 10(-8) mol of the thromboxane mimetic (15S)-hydroxyl-11 alpha,9 alpha-(epoxymethano)prosta-5Z,13E-dienoic acid (U-46619) was injected selectively into the tracheal arterial circulation, causing a transient contractile response (less than or equal to 10 g/cm). Additional frequency response studies were generated 7 min before and 1, 15, 30, 45, and 60 min after U-46619. Substantial augmentation of tracheal contraction to efferent vagal stimulation was observed after U-46619 for all frequencies greater than 4 Hz (P less than 0.02). Augmentation of vagally mediated contraction was not observed in four other dogs after equivalent tracheal contraction was elicited without U-46619. Similarly, in four separate dogs, augmentation of tracheal contraction was not observed when acetylcholine was given instead of vagal stimulation after U-46619. We conclude that the thromboxane analogue, U-46619, causes augmentation of tracheal contractile response induced by efferent vagus nerve stimulation. Potentiation is caused by a prejunctional action of U-46619 and is not induced by nonspecific precontraction with another agonist.

Duke Scholars

Published In

J Appl Physiol (1985)

DOI

ISSN

8750-7587

Publication Date

September 1986

Volume

61

Issue

3

Start / End Page

1173 / 1179

Location

United States

Related Subject Headings

  • Vagus Nerve
  • Trachea
  • Thromboxanes
  • Prostaglandin Endoperoxides, Synthetic
  • Physiology
  • Muscle Contraction
  • Male
  • Female
  • Electric Stimulation
  • Dogs
 

Citation

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Munoz, N. M., Shioya, T., Murphy, T. M., Primack, S., Dame, C., Sands, M. F., & Leff, A. R. (1986). Potentiation of vagal contractile response by thromboxane mimetic U-46619. J Appl Physiol (1985), 61(3), 1173–1179. https://doi.org/10.1152/jappl.1986.61.3.1173
Munoz, N. M., T. Shioya, T. M. Murphy, S. Primack, C. Dame, M. F. Sands, and A. R. Leff. “Potentiation of vagal contractile response by thromboxane mimetic U-46619.J Appl Physiol (1985) 61, no. 3 (September 1986): 1173–79. https://doi.org/10.1152/jappl.1986.61.3.1173.
Munoz NM, Shioya T, Murphy TM, Primack S, Dame C, Sands MF, et al. Potentiation of vagal contractile response by thromboxane mimetic U-46619. J Appl Physiol (1985). 1986 Sep;61(3):1173–9.
Munoz, N. M., et al. “Potentiation of vagal contractile response by thromboxane mimetic U-46619.J Appl Physiol (1985), vol. 61, no. 3, Sept. 1986, pp. 1173–79. Pubmed, doi:10.1152/jappl.1986.61.3.1173.
Munoz NM, Shioya T, Murphy TM, Primack S, Dame C, Sands MF, Leff AR. Potentiation of vagal contractile response by thromboxane mimetic U-46619. J Appl Physiol (1985). 1986 Sep;61(3):1173–1179.

Published In

J Appl Physiol (1985)

DOI

ISSN

8750-7587

Publication Date

September 1986

Volume

61

Issue

3

Start / End Page

1173 / 1179

Location

United States

Related Subject Headings

  • Vagus Nerve
  • Trachea
  • Thromboxanes
  • Prostaglandin Endoperoxides, Synthetic
  • Physiology
  • Muscle Contraction
  • Male
  • Female
  • Electric Stimulation
  • Dogs