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Characterization of skin cytokines in bullous pemphigoid and pemphigus vulgaris.

Publication ,  Journal Article
Rico, MJ; Benning, C; Weingart, ES; Streilein, RD; Hall, RP
Published in: Br J Dermatol
June 1999

The purpose of this study was to determine cytokine and cell marker expression in perilesional skin biopsies from patients with the autoimmune blistering diseases bullous pemphigoid (BP, n = 21) and pemphigus vulgaris (PV, n = 7). Immunohistochemistry and in situ hybridization were used to detect T helper (Th)1 [interleukin (IL)-2, interferon (IFN)-gamma] and Th2 (IL-4, IL-5, IL-13) protein and mRNA. Perilesional skin biopsies from patients with BP were characterized by the deposition of IL-4, IL-13 and IL-5. In patients with BP, IL-4 and IL-13 localized to mononuclear cells within the dermal infiltrate while IL-5 was predominately expressed at the dermal-epidermal junction. BP skin sections also expressed vascular cell adhesion molecule 1 on endothelial cells, not seen in patients with PV. PV biopsies were remarkable for a mixed Th1/Th2 pattern of cytokine expression, including the presence of IL-2, IFN-gamma and IL-4 and the absence of IL-5 and IL-13. In situ hybridization detected mRNA for IL-4 and IL-5 in the cellular infiltrate of BP patients, and IL-2 in a patient with PV. In vitro binding assays demonstrated that normal human eosinophils, activated by coculture in IL-5, bound preferentially to BP skin sections that contained detectable in vivo bound IL-5. The predominance of Th2 cytokines in BP, in association with increased binding of eosinophils in vitro, suggests that Th2 cytokines are relevant in the recruitment and adhesion of eosinophils within the dermal infiltrates of patients with BP, and may play a part in the pathogenesis of blister formation.

Duke Scholars

Published In

Br J Dermatol

DOI

ISSN

0007-0963

Publication Date

June 1999

Volume

140

Issue

6

Start / End Page

1079 / 1086

Location

England

Related Subject Headings

  • Vascular Cell Adhesion Molecule-1
  • Skin
  • RNA, Messenger
  • Pemphigus
  • Pemphigoid, Bullous
  • Interleukin-5
  • Interleukin-4
  • Interleukin-2
  • Interleukin-13
  • Interferon-gamma
 

Citation

APA
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ICMJE
MLA
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Rico, M. J., Benning, C., Weingart, E. S., Streilein, R. D., & Hall, R. P. (1999). Characterization of skin cytokines in bullous pemphigoid and pemphigus vulgaris. Br J Dermatol, 140(6), 1079–1086. https://doi.org/10.1046/j.1365-2133.1999.02907.x
Rico, M. J., C. Benning, E. S. Weingart, R. D. Streilein, and R. P. Hall. “Characterization of skin cytokines in bullous pemphigoid and pemphigus vulgaris.Br J Dermatol 140, no. 6 (June 1999): 1079–86. https://doi.org/10.1046/j.1365-2133.1999.02907.x.
Rico MJ, Benning C, Weingart ES, Streilein RD, Hall RP. Characterization of skin cytokines in bullous pemphigoid and pemphigus vulgaris. Br J Dermatol. 1999 Jun;140(6):1079–86.
Rico, M. J., et al. “Characterization of skin cytokines in bullous pemphigoid and pemphigus vulgaris.Br J Dermatol, vol. 140, no. 6, June 1999, pp. 1079–86. Pubmed, doi:10.1046/j.1365-2133.1999.02907.x.
Rico MJ, Benning C, Weingart ES, Streilein RD, Hall RP. Characterization of skin cytokines in bullous pemphigoid and pemphigus vulgaris. Br J Dermatol. 1999 Jun;140(6):1079–1086.
Journal cover image

Published In

Br J Dermatol

DOI

ISSN

0007-0963

Publication Date

June 1999

Volume

140

Issue

6

Start / End Page

1079 / 1086

Location

England

Related Subject Headings

  • Vascular Cell Adhesion Molecule-1
  • Skin
  • RNA, Messenger
  • Pemphigus
  • Pemphigoid, Bullous
  • Interleukin-5
  • Interleukin-4
  • Interleukin-2
  • Interleukin-13
  • Interferon-gamma