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Characterization of autoantibodies in pemphigus using antigen-specific enzyme-linked immunosorbent assays with baculovirus-expressed recombinant desmogleins.

Publication ,  Journal Article
Ishii, K; Amagai, M; Hall, RP; Hashimoto, T; Takayanagi, A; Gamou, S; Shimizu, N; Nishikawa, T
Published in: J Immunol
August 15, 1997

Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are autoimmune skin diseases caused by autoantibodies against desmoglein (Dsg) 3 and Dsg1, respectively. Routine immunofluorescence testing of skin and serum from patients cannot distinguish between these two severe diseases since both have IgG Abs directed against keratinocyte cell surfaces. In this study, recombinant Dsg3 and Dsg1, produced as secreted proteins by baculovirus expression, have been utilized to develop ELISAs for the specific characterization of their autoantibodies. Of 49 PV sera, 46 were positive in the Dsg3 ELISA and 44 of 46 PF sera were positive in the Dsg1 ELISA, compared with only 3 of 23 sera of bullous pemphigoid, and none of 53 normal control sera in both ELISAs. Both the Dsg3 and Dsg1 ELISAs were more specific and sensitive than conventional immunofluorescence staining. These Ag-specific ELISAs revealed that more than one-half of PV sera (26 of 49) had anti-Dsg1 Abs in addition to anti-Dsg3 Abs. PV patients who had not only oral mucous lesions but also significant skin involvement tended to have higher titers of anti-Dsg1 Abs. Furthermore, the ELISA reactivity correlated well with clinical disease activity in 5 of 6 PV and 5 of 5 PF patients. This ELISA provides a sensitive and highly specific assay for the diagnosis of patients with PV and PF, the correlation of disease activity with serum Ab levels, and a novel tool for investigating the immunopathogenesis of pemphigus.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

August 15, 1997

Volume

159

Issue

4

Start / End Page

2010 / 2017

Location

United States

Related Subject Headings

  • Recombinant Proteins
  • Pemphigus
  • Immunology
  • Immunoglobulin G
  • Humans
  • Enzyme-Linked Immunosorbent Assay
  • Desmoplakins
  • Desmogleins
  • Desmoglein 1
  • Cytoskeletal Proteins
 

Citation

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Ishii, K., Amagai, M., Hall, R. P., Hashimoto, T., Takayanagi, A., Gamou, S., … Nishikawa, T. (1997). Characterization of autoantibodies in pemphigus using antigen-specific enzyme-linked immunosorbent assays with baculovirus-expressed recombinant desmogleins. J Immunol, 159(4), 2010–2017.
Ishii, K., M. Amagai, R. P. Hall, T. Hashimoto, A. Takayanagi, S. Gamou, N. Shimizu, and T. Nishikawa. “Characterization of autoantibodies in pemphigus using antigen-specific enzyme-linked immunosorbent assays with baculovirus-expressed recombinant desmogleins.J Immunol 159, no. 4 (August 15, 1997): 2010–17.
Ishii K, Amagai M, Hall RP, Hashimoto T, Takayanagi A, Gamou S, et al. Characterization of autoantibodies in pemphigus using antigen-specific enzyme-linked immunosorbent assays with baculovirus-expressed recombinant desmogleins. J Immunol. 1997 Aug 15;159(4):2010–7.
Ishii K, Amagai M, Hall RP, Hashimoto T, Takayanagi A, Gamou S, Shimizu N, Nishikawa T. Characterization of autoantibodies in pemphigus using antigen-specific enzyme-linked immunosorbent assays with baculovirus-expressed recombinant desmogleins. J Immunol. 1997 Aug 15;159(4):2010–2017.

Published In

J Immunol

ISSN

0022-1767

Publication Date

August 15, 1997

Volume

159

Issue

4

Start / End Page

2010 / 2017

Location

United States

Related Subject Headings

  • Recombinant Proteins
  • Pemphigus
  • Immunology
  • Immunoglobulin G
  • Humans
  • Enzyme-Linked Immunosorbent Assay
  • Desmoplakins
  • Desmogleins
  • Desmoglein 1
  • Cytoskeletal Proteins