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Protection of mice from Semliki Forest virus infection by lymphocytes treated with low levels of interferon.

Publication ,  Journal Article
Stanton, GJ; Lloyd, RE; Sarzotti, M; Blalock, JE
Published in: Mol Biother
1989

These studies provide the first evidence that adoptive transfer of syngeneic mouse (BALB/c) lymphocytes treated with low levels of mouse interferon (IFN)-alpha/beta can result in sufficient protection to protect mice from Semliki Forest virus (SFV) infection. Specifically, intraperitoneal inoculation of noncytotoxic lymphocytes treated exogenously with IFN (3 to 50 U/ml), washed exhaustively, and mixed with antibody to IFN-alpha/beta to neutralize any residual or early produced IFN, resulted (after repeated studies) in a 35% to 40% reduction in mortality of mice challenged with SFV (P less than or equal to .01), while inoculation of control lymphocytes had no effect. Direct administration of relatively high levels of IFN-alpha/beta (2,000 U/d) only moderately reduced the mortality (by 20%) in mice. Passive transfer of IFN-treated BALB/c mouse embryo cells also did not protect. The protection could not be attributed to carryover of IFN by the lymphocytes, endogenous IFN induction, enhanced cytotoxicity of endogenous splenocytes or peritoneal leukocytes, or early appearance of antiviral neutralizing antibody. Thus, the most likely cause of the observed protection is consistent with a unique mechanism that can be activated by the IFN-treated lymphocytes.

Duke Scholars

Published In

Mol Biother

ISSN

0952-8172

Publication Date

1989

Volume

1

Issue

6

Start / End Page

305 / 310

Location

United States

Related Subject Headings

  • Togaviridae Infections
  • Semliki forest virus
  • Mice, Inbred BALB C
  • Mice
  • Lymphocytes
  • Leukocytes
  • Interferon Type I
  • Immunization, Passive
  • Embryo, Mammalian
  • Cytotoxicity, Immunologic
 

Citation

APA
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ICMJE
MLA
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Stanton, G. J., Lloyd, R. E., Sarzotti, M., & Blalock, J. E. (1989). Protection of mice from Semliki Forest virus infection by lymphocytes treated with low levels of interferon. Mol Biother, 1(6), 305–310.
Stanton, G. J., R. E. Lloyd, M. Sarzotti, and J. E. Blalock. “Protection of mice from Semliki Forest virus infection by lymphocytes treated with low levels of interferon.Mol Biother 1, no. 6 (1989): 305–10.
Stanton GJ, Lloyd RE, Sarzotti M, Blalock JE. Protection of mice from Semliki Forest virus infection by lymphocytes treated with low levels of interferon. Mol Biother. 1989;1(6):305–10.
Stanton, G. J., et al. “Protection of mice from Semliki Forest virus infection by lymphocytes treated with low levels of interferon.Mol Biother, vol. 1, no. 6, 1989, pp. 305–10.
Stanton GJ, Lloyd RE, Sarzotti M, Blalock JE. Protection of mice from Semliki Forest virus infection by lymphocytes treated with low levels of interferon. Mol Biother. 1989;1(6):305–310.

Published In

Mol Biother

ISSN

0952-8172

Publication Date

1989

Volume

1

Issue

6

Start / End Page

305 / 310

Location

United States

Related Subject Headings

  • Togaviridae Infections
  • Semliki forest virus
  • Mice, Inbred BALB C
  • Mice
  • Lymphocytes
  • Leukocytes
  • Interferon Type I
  • Immunization, Passive
  • Embryo, Mammalian
  • Cytotoxicity, Immunologic