Laser-excited sychronous flourescence spectrometer for benzo(a)pyrene

A laser-based synchronous scanning fluorimeter has been developed for the detection of polycyclic aromatic hydrocarbons (PAHs). Although extensive sample cleanup and separation are usually required, fluorescence has long been used for the determination of PAHs. The amount of sample pre-treatment can often be reduced when synchronous fluorescence (SF) is used, and the sensitivity of SF can be increased by the high power and narrow profile of a laser source. A small, portable, pulsed dye laser was used in this study. The instrument and its application to the determination of the carcinogen, benzo(a)pyrene, are described.

Duke Scholars

Author Tuan Vo-Dinh Biomedical Engineering