Use of transmitted room-temperature phosphorescence to improve nalidixic acid determination
A method for nalidixic acid (NA) determination by transmitted room-temperature phosphorescence is proposed. The antibacterial agent NA in acidic solution shows native fluorescence; when NA is spotted on a filter paper precoated with β-cyclodextrin and lead (II) acetate and dried for 5 min, phosphorescence results. The transmitted phosphorescence was measured directly with the use of a homemade sample holder. The excitation and emission wavelengths were 330 and 417 nm, respectively. The applicable concentration range was between 0.06 and 4.50 μg/mL with a relative standard deviation of 2.8% and a detection limit of 0.02 μg/mL. The method was applied to the determination of NA in human urine and pharmaceuticals, with recoveries from 97.5 to 105.5%.
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- Analytical Chemistry
- 0913 Mechanical Engineering
- 0306 Physical Chemistry (incl. Structural)
- 0301 Analytical Chemistry
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Analytical Chemistry
- 0913 Mechanical Engineering
- 0306 Physical Chemistry (incl. Structural)
- 0301 Analytical Chemistry