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Laser-induced fluorescence spectroscopy for skin cancer diagnostics

Publication ,  Journal Article
Panjehpour, M; Julius, C; Overholt, S; Phan, M; Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 2001

Objective: The goal of this pilot study was to determine whether in vivo laser-induced fluorescence intensity may be used to distinguish normal skin from malignant lesions. Materials and Methods: The system consisted of a nitrogen/dye laser tuned at 410 nm, an optical multichannel analyzer, and a fiber optic probe designed for excitation of tissue and collection of fluorescence emission. The intensity of fluorescence at the wavelength of 500 nm (I500) was used as the discriminating index. All data were normalized with respect to fluorescence of a standard dye. A total of 117 measurements were performed from normal and abnormal tissues in 28 patients. Patients were classified as having either of skin types I, II or III. Following the measurement, a biopsy of the abnormal tissue was performed. Using the biopsy results, each measurement was assigned under one of the following categories: basal cell carcinoma, squamous cell carcinoma, precancerous lesions, and benign lesions. Results: In patients with skin type I, the I500 index for basal cell carcinoma, squamous cell carcinoma and precancerous lesions were significantly different than that of normal tissue. There was no significant difference between I500 of benign lesions and normal tissue. In patients with skin type II, the I500 for basal cell carcinoma and squamous cell carcinoma was significantly different than that of normal tissues. Benign tissue could not be distinguished from normal tissue. In patients with skin type III, basal cell carcinoma and precancerous lesion could be easily distinguished from normal tissues. However, there was a statistically significant difference between benign and normal tissue. Conclusion: This pilot study indicated that the laser-induced fluorescence intensity of skin may be used to distinguish normal from cancerous and precancerous tissue.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

January 1, 2001

Volume

4254

Start / End Page

136 / 143

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Panjehpour, M., Julius, C., Overholt, S., Phan, M., & Vo-Dinh, T. (2001). Laser-induced fluorescence spectroscopy for skin cancer diagnostics. Proceedings of SPIE - The International Society for Optical Engineering, 4254, 136–143. https://doi.org/10.1117/12.427954
Panjehpour, M., C. Julius, S. Overholt, M. Phan, and T. Vo-Dinh. “Laser-induced fluorescence spectroscopy for skin cancer diagnostics.” Proceedings of SPIE - The International Society for Optical Engineering 4254 (January 1, 2001): 136–43. https://doi.org/10.1117/12.427954.
Panjehpour M, Julius C, Overholt S, Phan M, Vo-Dinh T. Laser-induced fluorescence spectroscopy for skin cancer diagnostics. Proceedings of SPIE - The International Society for Optical Engineering. 2001 Jan 1;4254:136–43.
Panjehpour, M., et al. “Laser-induced fluorescence spectroscopy for skin cancer diagnostics.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 4254, Jan. 2001, pp. 136–43. Scopus, doi:10.1117/12.427954.
Panjehpour M, Julius C, Overholt S, Phan M, Vo-Dinh T. Laser-induced fluorescence spectroscopy for skin cancer diagnostics. Proceedings of SPIE - The International Society for Optical Engineering. 2001 Jan 1;4254:136–143.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

January 1, 2001

Volume

4254

Start / End Page

136 / 143

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering