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Investigation of microfabrication of biological sample arrays using piezoelectric and bubble-jet printing technologies

Publication ,  Journal Article
Allain, LR; Stratis-Cullum, DN; Vo-Dinh, T
Published in: Analytica Chimica Acta
August 2, 2004

Modified commercial ink-jet printers are simple and cost-effective tools for the fabrication of microarrays containing biological samples. In this paper, the two most common ink-dispensing technologies, thermal and piezoelectric, were investigated, tested, and compared in terms of their ease of use and spotting quality. Ink cartridges were used as sample reservoirs and refilled with small volumes (as low as 20 μL) of water-based sample solutions containing herring sperm DNA and fluorescent dyes. Different solution compositions were evaluated and compared. Microscopic spots (<200 μm) were printed on various membranes (cellulose, nylon, nitrocellulose, and Zeta-Probe) and glass microscope slides. This technique enabled rapid preparation of a large number of printed arrays of biological materials at extremely low cost. To further demonstrate the utility of the microarray platform, we performed an assay of Bacillus anthracis, detected through DNA hybridization using fluorescent probes complementary to the B. anthracis target printed on a membrane. A comparison of Zeta-Probe and nylon membranes yielded less non-specific binding when using the Zeta-Probe membrane. In addition, detection of 100 fmol of the target per printed spot was demonstrated. © 2004 Published by Elsevier B.V.

Duke Scholars

Published In

Analytica Chimica Acta

DOI

ISSN

0003-2670

Publication Date

August 2, 2004

Volume

518

Issue

1-2

Start / End Page

77 / 85

Related Subject Headings

  • Analytical Chemistry
  • 4018 Nanotechnology
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 0399 Other Chemical Sciences
  • 0301 Analytical Chemistry
 

Citation

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ICMJE
MLA
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Allain, L. R., Stratis-Cullum, D. N., & Vo-Dinh, T. (2004). Investigation of microfabrication of biological sample arrays using piezoelectric and bubble-jet printing technologies. Analytica Chimica Acta, 518(1–2), 77–85. https://doi.org/10.1016/j.aca.2004.04.065
Allain, L. R., D. N. Stratis-Cullum, and T. Vo-Dinh. “Investigation of microfabrication of biological sample arrays using piezoelectric and bubble-jet printing technologies.” Analytica Chimica Acta 518, no. 1–2 (August 2, 2004): 77–85. https://doi.org/10.1016/j.aca.2004.04.065.
Allain LR, Stratis-Cullum DN, Vo-Dinh T. Investigation of microfabrication of biological sample arrays using piezoelectric and bubble-jet printing technologies. Analytica Chimica Acta. 2004 Aug 2;518(1–2):77–85.
Allain, L. R., et al. “Investigation of microfabrication of biological sample arrays using piezoelectric and bubble-jet printing technologies.” Analytica Chimica Acta, vol. 518, no. 1–2, Aug. 2004, pp. 77–85. Scopus, doi:10.1016/j.aca.2004.04.065.
Allain LR, Stratis-Cullum DN, Vo-Dinh T. Investigation of microfabrication of biological sample arrays using piezoelectric and bubble-jet printing technologies. Analytica Chimica Acta. 2004 Aug 2;518(1–2):77–85.
Journal cover image

Published In

Analytica Chimica Acta

DOI

ISSN

0003-2670

Publication Date

August 2, 2004

Volume

518

Issue

1-2

Start / End Page

77 / 85

Related Subject Headings

  • Analytical Chemistry
  • 4018 Nanotechnology
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 0399 Other Chemical Sciences
  • 0301 Analytical Chemistry