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Detection of bacterial pathogen DNA using an integrated complementary metal oxide semiconductor microchip system with capillary array electrophoresis.

Publication ,  Journal Article
Myong Song, J; Mobley, J; Vo-Dinh, T
Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
January 2003

In this paper, we show an integrated complementary metal oxide semiconductor (CMOS)-based microchip system with capillary array electrophoresis (CAE) for the detection of bacterial pathogen amplified by polymerase chain reaction (PCR). In order to demonstrate the efficacy of PCR reaction for the heat-labile toxin producing enterotoxigenic Escherichia coli (E. coli), which causes cholera-like diarrhea, 100 bp DNA ladders were injected along with the PCR product. Poly(vinylpyrrolidone) (PVP) was used as the separation medium and provided separation resolution which was adequate for the identification of PCR product. The miniaturized integrated CMOS microchip system with CAE has excellent advantages over conventional instrumental systems for analysis of bacterial pathogens such as compactness, low cost, high speed, and multiplex capability. Furthermore, the miniaturized integrated CMOS microchip system should be compatible with a variety of microfabricated devices that aim at more rapid and high-throughput analysis.

Duke Scholars

Published In

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI

EISSN

1873-376X

ISSN

1570-0232

Publication Date

January 2003

Volume

783

Issue

2

Start / End Page

501 / 508

Related Subject Headings

  • Semiconductors
  • Electrophoresis, Capillary
  • DNA, Bacterial
  • 3401 Analytical chemistry
  • 3214 Pharmacology and pharmaceutical sciences
  • 3101 Biochemistry and cell biology
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 0601 Biochemistry and Cell Biology
  • 0301 Analytical Chemistry
 

Citation

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ICMJE
MLA
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Myong Song, J., Mobley, J., & Vo-Dinh, T. (2003). Detection of bacterial pathogen DNA using an integrated complementary metal oxide semiconductor microchip system with capillary array electrophoresis. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 783(2), 501–508. https://doi.org/10.1016/s1570-0232(02)00747-x
Myong Song, Joon, Joel Mobley, and Tuan Vo-Dinh. “Detection of bacterial pathogen DNA using an integrated complementary metal oxide semiconductor microchip system with capillary array electrophoresis.Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 783, no. 2 (January 2003): 501–8. https://doi.org/10.1016/s1570-0232(02)00747-x.
Myong Song J, Mobley J, Vo-Dinh T. Detection of bacterial pathogen DNA using an integrated complementary metal oxide semiconductor microchip system with capillary array electrophoresis. Journal of chromatography B, Analytical technologies in the biomedical and life sciences. 2003 Jan;783(2):501–8.
Myong Song, Joon, et al. “Detection of bacterial pathogen DNA using an integrated complementary metal oxide semiconductor microchip system with capillary array electrophoresis.Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 783, no. 2, Jan. 2003, pp. 501–08. Epmc, doi:10.1016/s1570-0232(02)00747-x.
Myong Song J, Mobley J, Vo-Dinh T. Detection of bacterial pathogen DNA using an integrated complementary metal oxide semiconductor microchip system with capillary array electrophoresis. Journal of chromatography B, Analytical technologies in the biomedical and life sciences. 2003 Jan;783(2):501–508.
Journal cover image

Published In

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI

EISSN

1873-376X

ISSN

1570-0232

Publication Date

January 2003

Volume

783

Issue

2

Start / End Page

501 / 508

Related Subject Headings

  • Semiconductors
  • Electrophoresis, Capillary
  • DNA, Bacterial
  • 3401 Analytical chemistry
  • 3214 Pharmacology and pharmaceutical sciences
  • 3101 Biochemistry and cell biology
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 0601 Biochemistry and Cell Biology
  • 0301 Analytical Chemistry