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Subwavelength Raman imaging of biological samples using near-field spectroscopy

Publication ,  Journal Article
Deckert, V; Zeisel, D; Zenobi, R; Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 1999

Scanning near-field optical microscopy (SNOM) and Raman scattering were combined to obtain subwavelength molecular resolution. Near-field microscopy allows to overcome the diffraction limit valid for all lens or mirror based optical instruments and lateral resolutions well below 100 nm have been claimed. Raman spectroscopy yields information on the vibrational states of a molecule and therefore allows to distinguish between different chemical compounds easily. This is an advantage to the more widely used near-field fluorescence microscopy. To enhance the notoriously weak near-field Raman signal the sample was brought onto a Raman enhancing surface (silver coated Teflon nanospheres). Additionally brilliant cresyl blue (BCB) acted as a Raman label for our DNA samples. On such samples Raman images with a resolution better than 100 nm have been obtained. A single near-field SERS spectrum was measured in approximately 60 seconds. The acquisition time currently depends critically on the transmission of the near-field probes. Nevertheless, it is possible to measure whole near-field Raman images in a reasonable time. From these Raman images a preliminary distinction of different constitutions of adsorbed molecules can be done.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

ISSN

0277-786X

Publication Date

January 1, 1999

Volume

3607

Start / End Page

8 / 15

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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MLA
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Deckert, V., Zeisel, D., Zenobi, R., & Vo-Dinh, T. (1999). Subwavelength Raman imaging of biological samples using near-field spectroscopy. Proceedings of SPIE - The International Society for Optical Engineering, 3607, 8–15.
Deckert, V., D. Zeisel, R. Zenobi, and T. Vo-Dinh. “Subwavelength Raman imaging of biological samples using near-field spectroscopy.” Proceedings of SPIE - The International Society for Optical Engineering 3607 (January 1, 1999): 8–15.
Deckert V, Zeisel D, Zenobi R, Vo-Dinh T. Subwavelength Raman imaging of biological samples using near-field spectroscopy. Proceedings of SPIE - The International Society for Optical Engineering. 1999 Jan 1;3607:8–15.
Deckert, V., et al. “Subwavelength Raman imaging of biological samples using near-field spectroscopy.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 3607, Jan. 1999, pp. 8–15.
Deckert V, Zeisel D, Zenobi R, Vo-Dinh T. Subwavelength Raman imaging of biological samples using near-field spectroscopy. Proceedings of SPIE - The International Society for Optical Engineering. 1999 Jan 1;3607:8–15.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

ISSN

0277-786X

Publication Date

January 1, 1999

Volume

3607

Start / End Page

8 / 15

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering