Comparison of multichannel SIT image vidicon and photomultiplier sequential linear scanning systems for the measurement of steady-state and transient fluorescence of molecules in solution

The feasibility of using the SIT image vidicon as a potential detector for liquid chromatography is demonstrated. Limits of detection range from ~2×10-2 ng for anthracene to ~6×10-1 ng for riboflavin. A direct experimental comparison is made of a silicon intensified target vidicon multichannel (SIT-MC) and a photomultiplier sequential linear scanning (PM-SLS) system for the detection of steady-state fluorescence from molecules in solution. The PM system is slightly better than the SIT for measuring steady-state fluorescence; on the other hand, the multichannel advantage of the SIT would appear to make it the better system for measuring transient fluorescence signals

Duke Scholars

Author Tuan Vo-Dinh Biomedical Engineering