Development of a genosensor for Mycobacterium tuberculosis
Publication
, Journal Article
Isola, NR; Alarie, JP; Griffin, GD; Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 1996
This work involves the development of a fiberoptic genosensor for the detection of Mycobacterium tuberculosis. A rapid and sensitive non-radioactive method for the detection of Mycobacterium is described. This method involves polymerase chain reaction (PCR) amplification of the target DNA utilizing gene specific primers labelled with fluorescein at the 5' end and hybridization of the amplified product to a species specific oligonucleotide probe sequence covalently linked to the end of a fiber. The fluorescent hybridization products are detected by laser induced fluorescence using an argon laser (488 nm).
Duke Scholars
Published In
Proceedings of SPIE - The International Society for Optical Engineering
DOI
ISSN
0277-786X
Publication Date
January 1, 1996
Volume
2676
Start / End Page
228 / 240
Related Subject Headings
- 5102 Atomic, molecular and optical physics
- 4009 Electronics, sensors and digital hardware
- 4006 Communications engineering
Citation
APA
Chicago
ICMJE
MLA
NLM
Isola, N. R., Alarie, J. P., Griffin, G. D., & Vo-Dinh, T. (1996). Development of a genosensor for Mycobacterium tuberculosis. Proceedings of SPIE - The International Society for Optical Engineering, 2676, 228–240. https://doi.org/10.1117/12.238803
Isola, N. R., J. P. Alarie, G. D. Griffin, and T. Vo-Dinh. “Development of a genosensor for Mycobacterium tuberculosis.” Proceedings of SPIE - The International Society for Optical Engineering 2676 (January 1, 1996): 228–40. https://doi.org/10.1117/12.238803.
Isola NR, Alarie JP, Griffin GD, Vo-Dinh T. Development of a genosensor for Mycobacterium tuberculosis. Proceedings of SPIE - The International Society for Optical Engineering. 1996 Jan 1;2676:228–40.
Isola, N. R., et al. “Development of a genosensor for Mycobacterium tuberculosis.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 2676, Jan. 1996, pp. 228–40. Scopus, doi:10.1117/12.238803.
Isola NR, Alarie JP, Griffin GD, Vo-Dinh T. Development of a genosensor for Mycobacterium tuberculosis. Proceedings of SPIE - The International Society for Optical Engineering. 1996 Jan 1;2676:228–240.
Published In
Proceedings of SPIE - The International Society for Optical Engineering
DOI
ISSN
0277-786X
Publication Date
January 1, 1996
Volume
2676
Start / End Page
228 / 240
Related Subject Headings
- 5102 Atomic, molecular and optical physics
- 4009 Electronics, sensors and digital hardware
- 4006 Communications engineering