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Surface-enhanced Raman gene probe for HIV detection.

Publication ,  Journal Article
Isola, NR; Stokes, DL; Vo-Dinh, T
Published in: Analytical chemistry
April 1998

We report, for the first time, the use of surface-enhanced Raman (SERS)-active labels for primers used in polymerase chain reaction amplification of specific target DNA sequences. This method has the potential for combining the spectral selectivity and high sensitivity of the SERS technique with the inherent molecular specificity offered by DNA sequence hybridization. The effectiveness of the detection scheme is demonstrated using the gag gene sequence of the human immunodeficiency virus. The potential use of multiple probes for simultaneous detection of multiple biological targets is discussed.

Duke Scholars

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Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

April 1998

Volume

70

Issue

7

Start / End Page

1352 / 1356

Related Subject Headings

  • Surface Properties
  • Spectrum Analysis, Raman
  • Polymerase Chain Reaction
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Humans
  • HIV
  • Genes, gag
  • DNA, Viral
  • DNA Probes
 

Citation

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Isola, N. R., Stokes, D. L., & Vo-Dinh, T. (1998). Surface-enhanced Raman gene probe for HIV detection. Analytical Chemistry, 70(7), 1352–1356. https://doi.org/10.1021/ac970901z
Isola, N. R., D. L. Stokes, and T. Vo-Dinh. “Surface-enhanced Raman gene probe for HIV detection.Analytical Chemistry 70, no. 7 (April 1998): 1352–56. https://doi.org/10.1021/ac970901z.
Isola NR, Stokes DL, Vo-Dinh T. Surface-enhanced Raman gene probe for HIV detection. Analytical chemistry. 1998 Apr;70(7):1352–6.
Isola, N. R., et al. “Surface-enhanced Raman gene probe for HIV detection.Analytical Chemistry, vol. 70, no. 7, Apr. 1998, pp. 1352–56. Epmc, doi:10.1021/ac970901z.
Isola NR, Stokes DL, Vo-Dinh T. Surface-enhanced Raman gene probe for HIV detection. Analytical chemistry. 1998 Apr;70(7):1352–1356.
Journal cover image

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

April 1998

Volume

70

Issue

7

Start / End Page

1352 / 1356

Related Subject Headings

  • Surface Properties
  • Spectrum Analysis, Raman
  • Polymerase Chain Reaction
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Humans
  • HIV
  • Genes, gag
  • DNA, Viral
  • DNA Probes