Skip to main content

Nanosensors for the analysis of a single cell

Publication ,  Journal Article
Cullum, BM; Griffin, GD; Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 2001

Submicron fiber-optic biosensors have been developed and used to measure toxic chemicals within single cells. These sensors are fabricated by pulling the distal-end of an optical fiber to a diameter of less than one micron and coating them with antibodies to selectively bind the species of interest. This paper describes the use of these fibers to selectively measure the concentration of benzo[a]pyrene tetrol, a metabolite of benzo[a]pyrene, within individual cells of two different cell lines, human mammary carcinoma cells and rat liver epithelial cells. The results from these measurements have been used to evaluate the analytical figures of merit of these sensors such as detection limits, which were found to be 0.64 ± 0.17 × 10-11 M for BPT. In addition, measurements were performed both extracellularly and intracellularly, and an increase in variation of approximately 4% was determined for the intacellular measurements, relative to the extracellular measurements.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

January 1, 2001

Volume

4254

Start / End Page

35 / 40

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cullum, B. M., Griffin, G. D., & Vo-Dinh, T. (2001). Nanosensors for the analysis of a single cell. Proceedings of SPIE - The International Society for Optical Engineering, 4254, 35–40. https://doi.org/10.1117/12.427943
Cullum, B. M., G. D. Griffin, and T. Vo-Dinh. “Nanosensors for the analysis of a single cell.” Proceedings of SPIE - The International Society for Optical Engineering 4254 (January 1, 2001): 35–40. https://doi.org/10.1117/12.427943.
Cullum BM, Griffin GD, Vo-Dinh T. Nanosensors for the analysis of a single cell. Proceedings of SPIE - The International Society for Optical Engineering. 2001 Jan 1;4254:35–40.
Cullum, B. M., et al. “Nanosensors for the analysis of a single cell.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 4254, Jan. 2001, pp. 35–40. Scopus, doi:10.1117/12.427943.
Cullum BM, Griffin GD, Vo-Dinh T. Nanosensors for the analysis of a single cell. Proceedings of SPIE - The International Society for Optical Engineering. 2001 Jan 1;4254:35–40.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

January 1, 2001

Volume

4254

Start / End Page

35 / 40

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering