Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics
Publication
, Journal Article
McCain, ST; Gehm, ME; Wang, Y; Pitsianis, NP; Brady, DJ
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
June 29, 2006
We have designed and constructed a multimodal multiplex Raman spectrometer which uses multi-wavelength excitation to better detect signals in the presence of fluorescence by taking advantage of the shift-variance of the Raman signal with respect to excitation frequency. Coupled with partial-least-squares (PLS) regression, the technique applied to ethanol estimation in a tissue phantom achieves root-mean-squared-cross-validation errors (RMSCVE) of 9.2 mmol/L with a model formed with 2 principal components, compared to a single wavelength data set with equivalent energy where 7 principal components were used to achieve an RMSCVE of 39.1 mmol/L.
Duke Scholars
Published In
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
DOI
ISSN
1605-7422
Publication Date
June 29, 2006
Volume
6093
Citation
APA
Chicago
ICMJE
MLA
NLM
McCain, S. T., Gehm, M. E., Wang, Y., Pitsianis, N. P., & Brady, D. J. (2006). Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 6093. https://doi.org/10.1117/12.646755
McCain, S. T., M. E. Gehm, Y. Wang, N. P. Pitsianis, and D. J. Brady. “Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE 6093 (June 29, 2006). https://doi.org/10.1117/12.646755.
McCain ST, Gehm ME, Wang Y, Pitsianis NP, Brady DJ. Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2006 Jun 29;6093.
McCain, S. T., et al. “Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6093, June 2006. Scopus, doi:10.1117/12.646755.
McCain ST, Gehm ME, Wang Y, Pitsianis NP, Brady DJ. Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2006 Jun 29;6093.
Published In
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
DOI
ISSN
1605-7422
Publication Date
June 29, 2006
Volume
6093