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Structural basis for the root effect in haemoglobin.

Publication ,  Journal Article
Mylvaganam, SE; Bonaventura, C; Bonaventura, J; Getzoff, ED
Published in: Nature structural biology
March 1996

The remarkable ability of root effect haemoglobins to pump oxygen against high O2 gradients results from extreme, acid-induced reductions in O2 affinity and cooperativity. The long-sought mechanism for the root effect, revealed by the 2 angstrom crystal structure of the ligand-bound haemoglobin from Leiostomus xanthurus at pH 7.5, unexpectedly involves modulation of the R-state. Key residues strategically assemble positive-charge clusters across the allosteric beta1 beta2-interface in the R-state. At low pH, protonation of the beta N terminus and His 147(HC3)beta within these clusters is postulated to destabilize the R-state and promote the acid-triggered, allosteric R-->T switch with concomitant O2 release. Surprisingly, a set of residues specific to root effect haemoglobins recruit additional residues, conserved among most haemoglobins, to produce the root effect.

Duke Scholars

Published In

Nature structural biology

DOI

ISSN

1072-8368

Publication Date

March 1996

Volume

3

Issue

3

Start / End Page

275 / 283

Related Subject Headings

  • Species Specificity
  • Sequence Homology, Amino Acid
  • Molecular Sequence Data
  • Models, Molecular
  • Macromolecular Substances
  • Hydrogen-Ion Concentration
  • Humans
  • Hemoglobins
  • Fishes
  • Electrochemistry
 

Citation

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Mylvaganam, S. E., Bonaventura, C., Bonaventura, J., & Getzoff, E. D. (1996). Structural basis for the root effect in haemoglobin. Nature Structural Biology, 3(3), 275–283. https://doi.org/10.1038/nsb0396-275
Mylvaganam, S. E., C. Bonaventura, J. Bonaventura, and E. D. Getzoff. “Structural basis for the root effect in haemoglobin.Nature Structural Biology 3, no. 3 (March 1996): 275–83. https://doi.org/10.1038/nsb0396-275.
Mylvaganam SE, Bonaventura C, Bonaventura J, Getzoff ED. Structural basis for the root effect in haemoglobin. Nature structural biology. 1996 Mar;3(3):275–83.
Mylvaganam, S. E., et al. “Structural basis for the root effect in haemoglobin.Nature Structural Biology, vol. 3, no. 3, Mar. 1996, pp. 275–83. Epmc, doi:10.1038/nsb0396-275.
Mylvaganam SE, Bonaventura C, Bonaventura J, Getzoff ED. Structural basis for the root effect in haemoglobin. Nature structural biology. 1996 Mar;3(3):275–283.

Published In

Nature structural biology

DOI

ISSN

1072-8368

Publication Date

March 1996

Volume

3

Issue

3

Start / End Page

275 / 283

Related Subject Headings

  • Species Specificity
  • Sequence Homology, Amino Acid
  • Molecular Sequence Data
  • Models, Molecular
  • Macromolecular Substances
  • Hydrogen-Ion Concentration
  • Humans
  • Hemoglobins
  • Fishes
  • Electrochemistry