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High throughput detection of M6P/IGF2R intronic hypermethylation and LOH in ovarian cancer.

Publication ,  Journal Article
Huang, Z; Wen, Y; Shandilya, R; Marks, JR; Berchuck, A; Murphy, SK
Published in: Nucleic Acids Res
2006

Cell surface mannose 6-phosphate/insulin-like growth factor II receptors (M6P/IGF2R) bind and target exogenous insulin-like growth factor II (IGF2) to the prelysosomes where it is degraded. Loss of heterozygosity (LOH) for M6P/IGF2R is found in cancers, with mutational inactivation of the remaining allele. We exploited the normal allele-specific differential methylation of the M6P/IGF2R intron 2 CpG island to rapidly evaluate potential LOH in ovarian cancers, since every normal individual is informative. To this end, we developed a method for bisulfite modification of genomic DNA in 96-well format that allows for rapid methylation profiling. We identified ovarian cancers with M6P/IGF2R LOH, but unexpectedly also found frequent abnormal acquisition of methylation on the paternally inherited allele at intron 2. These results demonstrate the utility of our high-throughput method of bisulfite modification for analysis of large sample numbers. They further show that the methylation status of the intron 2 CpG island may be a useful indicator of LOH and biomarker of disease.

Duke Scholars

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

2006

Volume

34

Issue

2

Start / End Page

555 / 563

Location

England

Related Subject Headings

  • Sulfites
  • Sequence Analysis, DNA
  • Receptor, IGF Type 2
  • Polymerase Chain Reaction
  • Ovarian Neoplasms
  • Loss of Heterozygosity
  • Introns
  • Humans
  • Female
  • Developmental Biology
 

Citation

APA
Chicago
ICMJE
MLA
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Huang, Z., Wen, Y., Shandilya, R., Marks, J. R., Berchuck, A., & Murphy, S. K. (2006). High throughput detection of M6P/IGF2R intronic hypermethylation and LOH in ovarian cancer. Nucleic Acids Res, 34(2), 555–563. https://doi.org/10.1093/nar/gkj468
Huang, Zhiqing, Yaqing Wen, Ruby Shandilya, Jeffrey R. Marks, Andrew Berchuck, and Susan K. Murphy. “High throughput detection of M6P/IGF2R intronic hypermethylation and LOH in ovarian cancer.Nucleic Acids Res 34, no. 2 (2006): 555–63. https://doi.org/10.1093/nar/gkj468.
Huang Z, Wen Y, Shandilya R, Marks JR, Berchuck A, Murphy SK. High throughput detection of M6P/IGF2R intronic hypermethylation and LOH in ovarian cancer. Nucleic Acids Res. 2006;34(2):555–63.
Huang, Zhiqing, et al. “High throughput detection of M6P/IGF2R intronic hypermethylation and LOH in ovarian cancer.Nucleic Acids Res, vol. 34, no. 2, 2006, pp. 555–63. Pubmed, doi:10.1093/nar/gkj468.
Huang Z, Wen Y, Shandilya R, Marks JR, Berchuck A, Murphy SK. High throughput detection of M6P/IGF2R intronic hypermethylation and LOH in ovarian cancer. Nucleic Acids Res. 2006;34(2):555–563.
Journal cover image

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

2006

Volume

34

Issue

2

Start / End Page

555 / 563

Location

England

Related Subject Headings

  • Sulfites
  • Sequence Analysis, DNA
  • Receptor, IGF Type 2
  • Polymerase Chain Reaction
  • Ovarian Neoplasms
  • Loss of Heterozygosity
  • Introns
  • Humans
  • Female
  • Developmental Biology