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Flanking nuclear matrix attachment regions synergize with the T cell receptor delta enhancer to promote V(D)J recombination.

Publication ,  Journal Article
Zhong, XP; Carabaña, J; Krangel, MS
Published in: Proc Natl Acad Sci U S A
October 12, 1999

Previous studies have identified nuclear matrix attachment regions (MARs) that are closely associated with transcriptional enhancers in the IgH, Igkappa, and T cell receptor (TCR) beta loci, but have yielded conflicting information regarding their functional significance. In this report, a combination of in vitro and in situ mapping approaches was used to localize three MARs associated with the human TCR delta gene. Two of these are located within the Jdelta3-Cdelta intron, flanking the core TCR delta enhancer (Edelta) both 5' and 3' in a fashion reminiscent of the Ig heavy chain intronic enhancer-associated MARs. The third is located about 20 kb upstream, tightly linked to Ddelta1 and Ddelta2. We have previously used a transgenic minilocus V(D)J recombination reporter to establish that Edelta functions as a developmental regulator of V(D)J recombination, and that it does so by modulating substrate accessibility to the V(D)J recombinase. We show here that the Edelta-associated MARs function synergistically with the core Edelta to promote V(D)J recombination in this system, as they are required for enhancer-dependent transgene rearrangement in single-copy transgene integrants.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 12, 1999

Volume

96

Issue

21

Start / End Page

11970 / 11975

Location

United States

Related Subject Headings

  • VDJ Recombinases
  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, gamma-delta
  • Plasmids
  • Nuclear Matrix
  • Molecular Sequence Data
  • Models, Genetic
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
 

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Zhong, X. P., Carabaña, J., & Krangel, M. S. (1999). Flanking nuclear matrix attachment regions synergize with the T cell receptor delta enhancer to promote V(D)J recombination. Proc Natl Acad Sci U S A, 96(21), 11970–11975. https://doi.org/10.1073/pnas.96.21.11970
Zhong, X. P., J. Carabaña, and M. S. Krangel. “Flanking nuclear matrix attachment regions synergize with the T cell receptor delta enhancer to promote V(D)J recombination.Proc Natl Acad Sci U S A 96, no. 21 (October 12, 1999): 11970–75. https://doi.org/10.1073/pnas.96.21.11970.
Zhong XP, Carabaña J, Krangel MS. Flanking nuclear matrix attachment regions synergize with the T cell receptor delta enhancer to promote V(D)J recombination. Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11970–5.
Zhong, X. P., et al. “Flanking nuclear matrix attachment regions synergize with the T cell receptor delta enhancer to promote V(D)J recombination.Proc Natl Acad Sci U S A, vol. 96, no. 21, Oct. 1999, pp. 11970–75. Pubmed, doi:10.1073/pnas.96.21.11970.
Zhong XP, Carabaña J, Krangel MS. Flanking nuclear matrix attachment regions synergize with the T cell receptor delta enhancer to promote V(D)J recombination. Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11970–11975.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 12, 1999

Volume

96

Issue

21

Start / End Page

11970 / 11975

Location

United States

Related Subject Headings

  • VDJ Recombinases
  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, gamma-delta
  • Plasmids
  • Nuclear Matrix
  • Molecular Sequence Data
  • Models, Genetic
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice