Skip to main content

Regulation of T cell receptor beta allelic exclusion at a level beyond accessibility.

Publication ,  Journal Article
Jackson, A; Kondilis, HD; Khor, B; Sleckman, BP; Krangel, MS
Published in: Nat Immunol
February 2005

Allelic exclusion of V(beta)-to-DJ(beta) recombination depends on asynchronous rearrangement of alleles of the gene encoding T cell receptor beta in double-negative thymocytes and feedback inhibition that is maintained in double-positive thymocytes. Feedback is thought to be enforced through downregulation of V(beta) accessibility. In an attempt to override this negative regulation, we introduced the enhancer of the gene encoding T cell receptor alpha into the V(beta) gene cluster downstream of V(beta)12. In double-negative thymocytes, the introduced enhancer had no measurable effect on accessibility, but V(beta)12 rearrangement was stimulated and V(beta)12 allelic exclusion was partially subverted. In contrast, double-positive thymocytes showed increased V(beta) transcription and accessibility, but feedback inhibition of V(beta)-to-DJ(beta) recombination remained intact. Our results indicate additional regulatory constraints on V(beta)-to-DJ(beta) recombination that operate beyond the accessibility barrier.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nat Immunol

DOI

ISSN

1529-2908

Publication Date

February 2005

Volume

6

Issue

2

Start / End Page

189 / 197

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Thymus Gland
  • Receptors, Antigen, T-Cell, alpha-beta
  • Nucleosomes
  • Mice, Knockout
  • Mice
  • Immunology
  • Histones
  • Gene Rearrangement, T-Lymphocyte
  • Chromatin
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jackson, A., Kondilis, H. D., Khor, B., Sleckman, B. P., & Krangel, M. S. (2005). Regulation of T cell receptor beta allelic exclusion at a level beyond accessibility. Nat Immunol, 6(2), 189–197. https://doi.org/10.1038/ni1157
Jackson, Annette, Hrisavgi D. Kondilis, Bernard Khor, Barry P. Sleckman, and Michael S. Krangel. “Regulation of T cell receptor beta allelic exclusion at a level beyond accessibility.Nat Immunol 6, no. 2 (February 2005): 189–97. https://doi.org/10.1038/ni1157.
Jackson A, Kondilis HD, Khor B, Sleckman BP, Krangel MS. Regulation of T cell receptor beta allelic exclusion at a level beyond accessibility. Nat Immunol. 2005 Feb;6(2):189–97.
Jackson, Annette, et al. “Regulation of T cell receptor beta allelic exclusion at a level beyond accessibility.Nat Immunol, vol. 6, no. 2, Feb. 2005, pp. 189–97. Pubmed, doi:10.1038/ni1157.
Jackson A, Kondilis HD, Khor B, Sleckman BP, Krangel MS. Regulation of T cell receptor beta allelic exclusion at a level beyond accessibility. Nat Immunol. 2005 Feb;6(2):189–197.

Published In

Nat Immunol

DOI

ISSN

1529-2908

Publication Date

February 2005

Volume

6

Issue

2

Start / End Page

189 / 197

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Thymus Gland
  • Receptors, Antigen, T-Cell, alpha-beta
  • Nucleosomes
  • Mice, Knockout
  • Mice
  • Immunology
  • Histones
  • Gene Rearrangement, T-Lymphocyte
  • Chromatin