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Role for rearranged variable gene segments in directing secondary T cell receptor alpha recombination.

Publication ,  Journal Article
Hawwari, A; Krangel, MS
Published in: Proc Natl Acad Sci U S A
January 16, 2007

During the recombination of variable (V) and joining (J) gene segments at the T cell receptor alpha locus, a ValphaJalpha joint resulting from primary rearrangement can be replaced by subsequent rounds of secondary rearrangement that use progressively more 5' Valpha segments and progressively more 3' Jalpha segments. To understand the mechanisms that target secondary T cell receptor alpha recombination, we studied the behavior of a T cell receptor alpha allele (HYalpha) engineered to mimic a natural primary rearrangement of TRAV17 to Jalpha57. The introduced ValphaJalpha segment was shown to provide chromatin accessibility to Jalpha segments situated within several kilobases downstream and to suppress germ-line Jalpha promoter activity and accessibility at greater distances. As a consequence, the ValphaJalpha segment directed secondary recombination events to a subset of Jalpha segments immediately downstream from the primary rearrangement. The data provide the mechanistic basis for a model of primary and secondary T cell receptor alpha recombination in which recombination events progress in multiple small steps down the Jalpha array.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

January 16, 2007

Volume

104

Issue

3

Start / End Page

903 / 907

Location

United States

Related Subject Headings

  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, alpha-beta
  • Mice
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
  • Animals
  • Alleles
 

Citation

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MLA
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Hawwari, A., & Krangel, M. S. (2007). Role for rearranged variable gene segments in directing secondary T cell receptor alpha recombination. Proc Natl Acad Sci U S A, 104(3), 903–907. https://doi.org/10.1073/pnas.0608248104
Hawwari, Abbas, and Michael S. Krangel. “Role for rearranged variable gene segments in directing secondary T cell receptor alpha recombination.Proc Natl Acad Sci U S A 104, no. 3 (January 16, 2007): 903–7. https://doi.org/10.1073/pnas.0608248104.
Hawwari A, Krangel MS. Role for rearranged variable gene segments in directing secondary T cell receptor alpha recombination. Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):903–7.
Hawwari, Abbas, and Michael S. Krangel. “Role for rearranged variable gene segments in directing secondary T cell receptor alpha recombination.Proc Natl Acad Sci U S A, vol. 104, no. 3, Jan. 2007, pp. 903–07. Pubmed, doi:10.1073/pnas.0608248104.
Hawwari A, Krangel MS. Role for rearranged variable gene segments in directing secondary T cell receptor alpha recombination. Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):903–907.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

January 16, 2007

Volume

104

Issue

3

Start / End Page

903 / 907

Location

United States

Related Subject Headings

  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, alpha-beta
  • Mice
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
  • Animals
  • Alleles