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Protein phosphorylation responses in normal and cystic fibrosis airway epithelial cell lines.

Publication ,  Journal Article
Cohn, JA; Kole, J; Yankaskas, JR
Published in: Am J Respir Cell Mol Biol
October 1993

Monolayers of SV-40 immortalized human airway epithelial cell lines were stimulated with bradykinin and isoproterenol to study protein phosphorylation responses that accompany ion transport regulation in normal (BEAS) and cystic fibrosis (CF/T43) cells. Phosphorylation responses were analyzed by two-dimensional gel electrophoresis of postmicrosomal supernatant fractions of 32Pi-labeled cells. Isoproterenol increased the labeling of three phosphoproteins of M(r) 17,000, 18,000, and 37,000 that were equivalent to proteins known to undergo cAMP-dependent phosphorylation in T84 cell monolayers. Distinct proteins showed increased phosphorylation with bradykinin, including acidic proteins of M(r) 15,000 and 29,000. These resembled proteins exhibiting Ca(2+)-dependent phosphorylation T84 cells. The CF/T43 and BEAS cell protein phosphorylation responses were indistinguishable. These findings support the concept that the regulation and function of protein kinase A is normal in cystic fibrosis airway epithelia and that the abnormal cAMP-mediated regulation of chloride permeability in these cells is due to altered regulatory or effector proteins.

Duke Scholars

Published In

Am J Respir Cell Mol Biol

DOI

ISSN

1044-1549

Publication Date

October 1993

Volume

9

Issue

4

Start / End Page

401 / 404

Location

United States

Related Subject Headings

  • Trachea
  • Respiratory System
  • Phosphorylation
  • Humans
  • Epithelium
  • Epithelial Cells
  • Electrophoresis, Gel, Two-Dimensional
  • Cystic Fibrosis
  • Cell Line
  • 3201 Cardiovascular medicine and haematology
 

Citation

APA
Chicago
ICMJE
MLA
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Cohn, J. A., Kole, J., & Yankaskas, J. R. (1993). Protein phosphorylation responses in normal and cystic fibrosis airway epithelial cell lines. Am J Respir Cell Mol Biol, 9(4), 401–404. https://doi.org/10.1165/ajrcmb/9.4.401
Cohn, J. A., J. Kole, and J. R. Yankaskas. “Protein phosphorylation responses in normal and cystic fibrosis airway epithelial cell lines.Am J Respir Cell Mol Biol 9, no. 4 (October 1993): 401–4. https://doi.org/10.1165/ajrcmb/9.4.401.
Cohn JA, Kole J, Yankaskas JR. Protein phosphorylation responses in normal and cystic fibrosis airway epithelial cell lines. Am J Respir Cell Mol Biol. 1993 Oct;9(4):401–4.
Cohn, J. A., et al. “Protein phosphorylation responses in normal and cystic fibrosis airway epithelial cell lines.Am J Respir Cell Mol Biol, vol. 9, no. 4, Oct. 1993, pp. 401–04. Pubmed, doi:10.1165/ajrcmb/9.4.401.
Cohn JA, Kole J, Yankaskas JR. Protein phosphorylation responses in normal and cystic fibrosis airway epithelial cell lines. Am J Respir Cell Mol Biol. 1993 Oct;9(4):401–404.

Published In

Am J Respir Cell Mol Biol

DOI

ISSN

1044-1549

Publication Date

October 1993

Volume

9

Issue

4

Start / End Page

401 / 404

Location

United States

Related Subject Headings

  • Trachea
  • Respiratory System
  • Phosphorylation
  • Humans
  • Epithelium
  • Epithelial Cells
  • Electrophoresis, Gel, Two-Dimensional
  • Cystic Fibrosis
  • Cell Line
  • 3201 Cardiovascular medicine and haematology