Skip to main content

Cyclooxygenase-2 (COX-2) levels before and after chemotherapy: a study in rectal cancer.

Publication ,  Journal Article
Watwe, V; Javle, M; Lawrence, D; Groth, J; Iyer, R; El-Hajjar, D; Geradts, J
Published in: Am J Clin Oncol
December 2005

OBJECTIVES: Induction of cyclooxygenase-2 (COX-2) by inflammatory mediators, oncogenes, and carcinogens has been demonstrated in preclinical models. However, there are limited clinical data regarding COX-2 induction by chemotherapy or radiation. Experimental data suggest cross-talk between the EGFR and COX-2 pathways. The aim of this study was to analyze the expression of COX-2 before and after chemoradiation (CRT) and correlate the same with tumor (T) down-staging and survival. Similar data were obtained for EGFR expression before and after chemoradiation. METHODS: Archival paraffin-embedded tumor specimens from patients undergoing CRT between 1995 and 2001 were analyzed. COX-2 expression was measured by immunohistochemistry (IHC), using the 160112 COX-2 mouse monoclonal antibody. For EGFR, we used mouse monoclonal Ab-10. Standard immunoperoxidase technique was used to detect the avidin- biotin peroxidase complex. Staining in tumor tissue was visually scored and confirmed by an image analyzer (ACIS; ChromaVision Medical Systems, Inc, San Juan Capistrano, CA). RESULTS: Twenty pretreatment biopsy samples from rectal cancer patients and their paired, post-CRT surgical specimens (n = 17) were analyzed. Three cases had no primary tumor after CRT. COX-2 expression was noted in 19 of 20 pretreatment samples and 17 of 17 surgical specimens. EGFR expression was noted in 10 cases pretreatment. Six patients with weakly positive COX-2 expression pretreatment had increased COX-2 expression after CRT, whereas in 1 patient the expression decreased after CRT. No EGFR induction was noted. There was no statistical association between EGFR and COX-2 expression in this data set. Median survival for the entire cohort was 38.9 months. There was no difference in survival between the COX-2 induced and noninduced groups. CONCLUSIONS: COX-2 induction was seen with CRT in this population of rectal cancer patients. Prognostic significance of this induction remains to be defined in a larger cohort.

Duke Scholars

Published In

Am J Clin Oncol

DOI

EISSN

1537-453X

Publication Date

December 2005

Volume

28

Issue

6

Start / End Page

560 / 564

Location

United States

Related Subject Headings

  • Survival Analysis
  • Rectal Neoplasms
  • Proportional Hazards Models
  • Prognosis
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Middle Aged
  • Membrane Proteins
  • Male
  • Life Tables
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Watwe, V., Javle, M., Lawrence, D., Groth, J., Iyer, R., El-Hajjar, D., & Geradts, J. (2005). Cyclooxygenase-2 (COX-2) levels before and after chemotherapy: a study in rectal cancer. Am J Clin Oncol, 28(6), 560–564. https://doi.org/10.1097/01.coc.0000182476.34375.17
Watwe, Veena, Milind Javle, David Lawrence, Jeffrey Groth, Renuka Iyer, Dany El-Hajjar, and Joseph Geradts. “Cyclooxygenase-2 (COX-2) levels before and after chemotherapy: a study in rectal cancer.Am J Clin Oncol 28, no. 6 (December 2005): 560–64. https://doi.org/10.1097/01.coc.0000182476.34375.17.
Watwe V, Javle M, Lawrence D, Groth J, Iyer R, El-Hajjar D, et al. Cyclooxygenase-2 (COX-2) levels before and after chemotherapy: a study in rectal cancer. Am J Clin Oncol. 2005 Dec;28(6):560–4.
Watwe, Veena, et al. “Cyclooxygenase-2 (COX-2) levels before and after chemotherapy: a study in rectal cancer.Am J Clin Oncol, vol. 28, no. 6, Dec. 2005, pp. 560–64. Pubmed, doi:10.1097/01.coc.0000182476.34375.17.
Watwe V, Javle M, Lawrence D, Groth J, Iyer R, El-Hajjar D, Geradts J. Cyclooxygenase-2 (COX-2) levels before and after chemotherapy: a study in rectal cancer. Am J Clin Oncol. 2005 Dec;28(6):560–564.

Published In

Am J Clin Oncol

DOI

EISSN

1537-453X

Publication Date

December 2005

Volume

28

Issue

6

Start / End Page

560 / 564

Location

United States

Related Subject Headings

  • Survival Analysis
  • Rectal Neoplasms
  • Proportional Hazards Models
  • Prognosis
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Middle Aged
  • Membrane Proteins
  • Male
  • Life Tables