The rapid and large scale separation of peritoneal macrophages.
Publication
, Journal Article
Shaipanich, T; Cianciolo, GJ
Published in: Immunology
April 1972
A simple method for rapid and large scale (over 109 cells) separation of peritoneal macrophages by incubation in a glass-bead column and elution with buffered ethylenediamine tetraacetic acid (EDTA) solution is described. Macrophages obtained were about 90 per cent pure with the recovery rates of 38–53 per cent depending upon the sample size, and 83 per cent viability. Lymphocyte contamination was reduced to 5 per cent or less.
Duke Scholars
Published In
Immunology
ISSN
0019-2805
Publication Date
April 1972
Volume
22
Issue
4
Start / End Page
525 / 529
Location
England
Related Subject Headings
- Rats
- Peritoneum
- Methods
- Macrophages
- In Vitro Techniques
- Immunology
- Glass
- Edetic Acid
- Chromatography
- Animals
Citation
APA
Chicago
ICMJE
MLA
NLM
Shaipanich, T., & Cianciolo, G. J. (1972). The rapid and large scale separation of peritoneal macrophages. Immunology, 22(4), 525–529.
Shaipanich, T., and G. J. Cianciolo. “The rapid and large scale separation of peritoneal macrophages.” Immunology 22, no. 4 (April 1972): 525–29.
Shaipanich T, Cianciolo GJ. The rapid and large scale separation of peritoneal macrophages. Immunology. 1972 Apr;22(4):525–9.
Shaipanich, T., and G. J. Cianciolo. “The rapid and large scale separation of peritoneal macrophages.” Immunology, vol. 22, no. 4, Apr. 1972, pp. 525–29.
Shaipanich T, Cianciolo GJ. The rapid and large scale separation of peritoneal macrophages. Immunology. 1972 Apr;22(4):525–529.
Published In
Immunology
ISSN
0019-2805
Publication Date
April 1972
Volume
22
Issue
4
Start / End Page
525 / 529
Location
England
Related Subject Headings
- Rats
- Peritoneum
- Methods
- Macrophages
- In Vitro Techniques
- Immunology
- Glass
- Edetic Acid
- Chromatography
- Animals